評估藥物於治療粥狀動脈硬化的功效之方法

CNU IR > Pharmacy and Science > Dept. of Biotechnology (including master's program) > Patent > Item 310902800/30297

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/30297

Title:	評估藥物於治療粥狀動脈硬化的功效之方法 Method of Assessing Drug Efficacy In Treating Atherosclerosis
Authors:	郭玫君吳明娟王芷珊
Contributors:	生物科技系
Date:	2016-08-01
Issue Date:	2017-04-27 15:34:57 (UTC+8)
Abstract:	本發明係有關於一種評估藥物於治療粥狀動脈硬化的功效之方法，係先準備小鼠血管內皮細胞；加入腫瘤壞死因子於小鼠血管內皮細胞作用3~9個小時；再加入待測藥物於小鼠血管內皮細胞作用一段時間；以及利用複數引子組進行反轉?定?聚合??鎖反應（RT-qPCR）以偵測小鼠血管內皮細胞是否表現粥狀動脈硬化相關基因。 The invention relates to a method of assessing drug efficacy in treating atherosclerosis. The method comprises steps of preparing a mouse vascular endothelial cell; adding a tumor necrosis factor to the mouse vascular endothelial cell for reaction for 3-9 hours; adding a drug to be tested to the mouse vascular endothelial cell for reaction for a period of time; and detecting whether atherosclerosis related genes are expressed in the mouse vascular endothelial cell by using a plurality of primer sets to conduct RT-qPCR.
Relation:	公開號：201627668、專利編號：I570407 申請號：104101691 國際分類：G01N-033/15(2006.01);C12Q-001/68(2006.01);C40B-030/06(2006.01) 專利公報：14卷15期、44卷5期
Appears in Collections:	[Dept. of Biotechnology (including master's program)] Patent

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