本研究以類脂質體(Niosomes）之特殊結構，進行包覆去氧熊果素(Deoxy Abutin），比較使用不同界面活性劑Span 20(Sorbitan Monolaurate）及Span 60(Sorbitan Monostearate)，並添加不同比例之膽固醇(Cholesterol)，利用超音波震盪法及高壓均質乳化法(500 bar/10 cycles)兩種製程方法進行Niosomes的製備，並儲存於4℃、25℃及45℃中，進行粒徑大小、界面電位、安定性、包覆率以及經皮吸收之物性評估，其目的主要是為了避免及延緩活性成分的氧化及降解。實驗結果顯示，以界面活性劑Span 20製備之Niosomes，其平均粒徑大小、PdI值及界面電位值之結果皆比Span 60配方更穩定，因此本研究以Span 20進行深入探討。而經30天及90天安定性評估結果可知，相較於其他配方，以2.0% Span 20並在製備過程中添加0.5%膽固醇製備之配方，不論平均粒徑大小、電位值、PI值以及包覆率皆最穩定，總量回收率為100%，經皮吸收率為50%。以上結果皆證實，本研究製備之niosomes配方劑型與傳統乳液配方相比，能有效延緩去氧熊果素之洩漏及氧化降解之情形產生。 In this study, D-Arbutin encapsulated in niosomes by its special structure, the purpose of the present research in order to avoid oxidation and degradation of the active ingredient. This study choose two kinds of surfactants such as Sorbitan Monolaurate (Span 20) and Sorbitan Monostrearate (Span 60), and added the Cholesterol of different proportion then used sonication (80% Amp/20 min)/ high pressure homogenizers (500 bar/10 cycles) to employed for the preparation of niosomes. The stability and physical property investigation of niosomes were stored at 4℃, 25℃and 45℃, and to measure particle size, zeta-potential, encapsulation efficiency.The experimental results showed that the niosomes formulation NO.10 which was made by Span 20, particle size were around 200 nm and a narrow polydispersity index (PdI) lower than 0.2, the mean value of zeta potential were more than -30 mV. The encapsulation efficiency of the formulation NO.10 were more than 80%, then after 90 days, the stability test results showed that was best than another formulations. Finally, the results of the skin absorption showed that the formulation NO.10 significantly increased when added 0.1% ethanol in the solven solution.Therefore, the niosomes formulation NO.10 can effectively reduce leak and degradation of active ingredient, and it also can increase high chemical stability as compared with free drug.
