Dual role of CD44 isoforms in ampullary adenocarcinoma: CD44s predicts poor prognosis in early cancer and CD44. is an indicator for recurrence in advanced cancer

doi:10.1186/s12885-015-1924-3

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题名:	Dual role of CD44 isoforms in ampullary adenocarcinoma: CD44s predicts poor prognosis in early cancer and CD44. is an indicator for recurrence in advanced cancer
作者:	Wu, Cheng-Lin Chao, Ying-Jui Yang, Ta-Ming Chen, Yi-Ling Chang, Kung-Chao Hsu, Hui-Ping Shan, Yan-Shen Lai, Ming-Derg
贡献者:	老人服務事業管理系
关键词:	Ampullary cancer CD44 CD44ν3-10 CD44ν6-10 Ingenuity pathway analysis Pancreatic invasion
日期:	2015-11
上传时间:	2016-04-19 19:06:40 (UTC+8)
出版者:	Biomed Central Ltd
摘要:	Background: Although postoperative adjuvant chemoradiotherapies prevent recurrence for some patients with ampullary cancer, the recurrence rate is as high as 29 % in patients with stage I cancer. In an effort to identify predictors of recurrence in patients with ampullary adenocarcinoma, we investigated the clinical value of assessing standard and variant forms of CD44. Methods: Immunohistochemistry staining and reverse-transcription polymerase chain reaction (RT-PCR) was used to detect standard and variant forms of CD44 in samples of ampullary adenocarcinoma. The cDNA microarray analysis comparing tumors with or without pancreatic invasion was undertaken and analyzed by Ingenuity Pathway Analysis. Results: The standard CD44 (CD44s) isoform was detected in 76 of 98 patients with ampullary adenocarcinoma, and the negative or weak expression of CD44s was correlated with pancreatic invasion, lymphovascular invasion, advanced stage and bone metastasis. Moderate to dense expression of CD44s was correlated with shorter overall survival in patients with localized cancer (T1 or T2 disease, P = 0.0268). The patients with advanced cancer (T3 or T4 disease) and moderate or dense CD44s expression had a trend toward better survival. Alternative splicing of CD44 was confirmed using RT-PCR, which revealed that the CD44 nu 3-10 isoform was only expressed in patients with cancer recurrence. Fold change of CD44 nu 6-10 was also increased. In addition, networks containing CD44, vascular endothelial growth factor (VEGF), epidermal growth factor receptor (EGFR), transforming growth factor-beta (TGF-beta), matrix metalloproteinase 2 (MMP2), AKT, extracellular signal-regulated protein kinase 1 and 2 (ERK1/2), p38 MAPK, activated protein 1 (AP1), and CTNNB1 were constructed after comparing microarray data from patients with and without pancreatic invasion. Conclusions: Whereas CD44s functions as tumor-promoting oncoprotein in early localized ampullary adenocarcinoma, CD44 variants are expressed in advanced cancer and patients with recurrence. Regional invasiveness and distant metastasis of ampullary cancer is controlled by a complex interacting network.
關聯:	BMC Cancer, v.15, Article ID 903
显示于类别:	[Dept. of Senior Service and Health Management] Periodical Articles

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