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| 標題: | Cardio Protective Effects of Lumbrokinase and Dilong on Second-Hand Smoke-Induced Apoptotic Signaling in the Heart of a Rat Model |
| 作者: | Liao, Hung-En
Lai, Chao-Hung
Ho, Tsung-Jung
Yeh, Yu-Lan
Jong, Gwo-Ping
Kuo, Wu-Hsien
Chung, Li-Chin
Pai, Pei-ying
Wen, Su-Ying
Huang, Chih-Yang |
| 貢獻者: | 醫務管理系 |
| 關鍵字: | cardiac survival signaling
caspases
death-receptor-dependent pathway
dilong
lumbrokinase
mitochondria-dependent pathway
second-hand smoke (SHS) |
| 日期: | 2015-06 |
| 上傳時間: | 2016-04-19 19:06:15 (UTC+8) |
| 出版者: | Chinese Physiological Soc |
| 摘要: | Exposure to second-hand tobacco smoke (SHS) has been epidemiologically linked to heart disease among non-smokers. However, the molecular mechanism behind SHS-induced cardiac disease is not well known. This study found that SD rats exposed to cigarette smoke at a dose of 10 cigarettes for 30 min twice a day for 1 month had a reduced left ventricle-to-tibia length ratio (mg/mm), increased cardiomyocyte apoptosis by TUNEL assay and a wider interstitial space by H&E staining. However, lumbrokinase and dilong both reversed the effects of SHS. Western blotting demonstrated significantly increased expression of the pro-apoptotic protein caspase-3 in the hearts of the rats exposed to SHS. Elevated protein expression levels of Fas, FADD and the apoptotic initiator activated caspase-8, a molecule in the death-receptor-dependent pathway, coupled with increased t-Bid and apoptotic initiator activated caspase-9 were found. Molecules in the mitochondria-dependent pathway, which disrupts mitochondrial membrane potential, were also found in rats exposed to SHS. These factors indicate myocardial apoptosis. However, treatment with lumbrokinase and dilong inhibited SHS-induced apoptosis. Regarding regulation of the survival pathway, we found in western blot analysis that cardiac protein expression of pAkt, Bcl2, and Bcl-xL was significantly down-regulated in rats exposed to SHS. These effects were reversed with lumbrokinase and dilong treatment. The effects of SIB on cardiomyocytes were also found to be mediated by the Fas death receptor-dependent apoptotic pathway, an unbalanced mitochondria membrane potential and decreased survival signaling. However, treatment with both lumbrokinase and dilong inhibited the effects of SITS. Our data suggest that lumbrokinase and dilong may prevent heart disease in SITS-exposed non-smokers |
| 關聯: | Chinese Journal of Physiology, v.58 n.3, pp.188-196 |
| 顯示於類別: | [醫務管理系(所)] 期刊論文
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