Nerve Regeneration Potential of Protocatechuic Acid in RSC96 Schwann Cells by Induction of Cellular Proliferation and Migration through IGF-IR-PI3K-Akt Signaling

doi:10.4077/CJP.2015.BAD340

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標題:	Nerve Regeneration Potential of Protocatechuic Acid in RSC96 Schwann Cells by Induction of Cellular Proliferation and Migration through IGF-IR-PI3K-Akt Signaling
作者:	Ju, Da-Tong Liao, Hung-En Shibu, Marthandam Asokan Ho, Tsung-Jung Padma, Viswanadha Vijaya Tsai, Fuu-Jen Chung, Li-Chin Day, Cecilia Hsuan Lin, Chien-Chung Huang, Chih-Yang
貢獻者:	醫務管理系
關鍵字:	igf-i alpinia-oxyphylla cycleprogression phosphatidylinositol3-kinase growth-factors sciatic-nerve neurons apoptosis survival kinase
日期:	2015-12
上傳時間:	2016-04-19 19:05:17 (UTC+8)
出版者:	Chinese Physiological Soc
摘要:	Peripheral nerve injuries, caused by accidental trauma, acute compression or surgery, often result in temporary or life-long neuronal dysfunctions and inflict great economic or social burdens on the patients. Nerve cell proliferation is an essential process to restore injured nerves of adults. Schwann cells play a crucial role in endogenous repair of peripheral nerves due to their ability to proliferate, migrate and provide trophic support to axons via expression of various neurotrophic factors, such as the nerve growth factor (NGF), especially after nerve injury. Protocatechuic acid (PCA) is a dihydroxybenzoic acid, a type of phenolic acid, isolated from the kernels of Alpinia oxyphylla Miq (AOF), a traditional Chinese herbal medicine the fruits of which are widely used as a tonic, aphrodisiac, anti-salivation and anti-diarrheatic. This study investigated the molecular mechanisms by which PCA induces Schwann cell proliferation by activating IGF-IR-PI3K-Akt pathway. Treatment with PCA induces phosphorylation of the insulin-like growth factor-I (IGF-I)-mediated phosphatidylinositol 3 kinase/serine - threonine kinase (PI3K/Akt) pathway, and activates expression of cell nuclear antigen (PCNA) in a dose-dependent manner. Cell cycle analysis after 18 h of treatment showed that proliferation of the RSC96 cells was enhanced by PCA treatment. The PCA induced proliferation was accompanied by modulation in the expressions of cell cycle proteins cyclin D1, cyclin E and cyclin A. Knockdown of PI3K using small interfering RNA (siRNA) and inhibition of IGF-IR resulted in the reduction in cell survival proteins. The results collectively showed that PCA treatment promoted cell proliferation and cell survival via IGF-I signaling.
關聯:	Chinese Journal of Physiology, v.58 n.6, pp.412-419
顯示於類別:	[醫務管理系(所)] 期刊論文

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