Argininosuccinate synthetase 1 suppression and arginine restriction inhibit cell migration in gastric cancer cell lines

doi:10.1038/srep09783

CNU IR > College of Smart Living > Dept. of Senior Service and Health Management > Periodical Articles > Item 310902800/29698

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/29698

Title:	Argininosuccinate synthetase 1 suppression and arginine restriction inhibit cell migration in gastric cancer cell lines
Authors:	Shan, Yan-Shen Hsu, Hui-Ping Lai, Ming-Derg Yen, Meng-Chi Chen, Wei-Ching Fang, Jung-Hua Weng, Tzu-Yang Chen, Yi-Ling
Contributors:	老人服務事業管理系
Keywords:	Epithelial Ovarian-cancer Hepatocellular-Carcinoma Deiminase Treatment Signal Transducer Nitric-Oxide Tumor-Growth In-Vitro Expression Deprivation Stat3
Date:	2015-04
Issue Date:	2016-04-19 19:05:02 (UTC+8)
Publisher:	Nature Publishing Group
Abstract:	Gastric cancer metastasis remains a major cause of cancer-related deaths. There is an urgent need to develop new therapeutic approaches targeting metastatic gastric cancer. Argininosuccinate synthetase 1 (ASS1) expression is increased in gastric cancer. We detected the protein expression of ASS1 in human gastric cancer cell lines (AGS, NCI-N87, and MKN45) and in murine gastric cancer cell lines (3I and 3IB2). We used vector-mediated short hairpin RNA(shRNA) expression to silence ASS1 expression in the MKN45 and 3IB2 cell lines, and analyzed the effects of this protein on cell migration and metastasis. We demonstrated that ASS1 silencing suppressed cell migration in the MKN45 and 3IB2 cell lines. ASS1 knockdown significantly reduced liver metastasis in mice after the intrasplenic implantation of 3IB2 cancer cell clones. To determine whether arginine restriction may represent a therapeutic approach to treat gastric cancer, the sensitivity of tumor cells to arginine depletion was determined in gastric cancer cells. Arginine depletion significantly inhibited cell migration in the gastric cancer cell line. The silencing of ASS1 expression in MKN45 and 3IB2 gastric cancer cells markedly decreased STAT3 protein expression. In conclusion, our results indicate that the ASS1 protein is required for cell migration in gastric cancer cell lines.
Relation:	Scientific Reports, v.5 , Article ID 9783
Appears in Collections:	[Dept. of Senior Service and Health Management] Periodical Articles

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