Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/29698
English  |  正體中文  |  简体中文  |  Items with full text/Total items : 18268/20495 (89%)
Visitors : 8646713      Online Users : 998
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
    •  Adv. Search
    Home ‧ Login ‧ Upload ‧ Help ‧ About ‧ Administer Goto mobile version


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://ir.cnu.edu.tw/handle/310902800/29698


    题名: Argininosuccinate synthetase 1 suppression and arginine restriction inhibit cell migration in gastric cancer cell lines
    作者: Shan, Yan-Shen
    Hsu, Hui-Ping
    Lai, Ming-Derg
    Yen, Meng-Chi
    Chen, Wei-Ching
    Fang, Jung-Hua
    Weng, Tzu-Yang
    Chen, Yi-Ling
    贡献者: 老人服務事業管理系
    关键词: Epithelial Ovarian-cancer
    Hepatocellular-Carcinoma
    Deiminase Treatment
    Signal Transducer
    Nitric-Oxide
    Tumor-Growth
    In-Vitro
    Expression
    Deprivation
    Stat3
    日期: 2015-04
    上传时间: 2016-04-19 19:05:02 (UTC+8)
    出版者: Nature Publishing Group
    摘要: Gastric cancer metastasis remains a major cause of cancer-related deaths. There is an urgent need to develop new therapeutic approaches targeting metastatic gastric cancer. Argininosuccinate synthetase 1 (ASS1) expression is increased in gastric cancer. We detected the protein expression of ASS1 in human gastric cancer cell lines (AGS, NCI-N87, and MKN45) and in murine gastric cancer cell lines (3I and 3IB2). We used vector-mediated short hairpin RNA(shRNA) expression to silence ASS1 expression in the MKN45 and 3IB2 cell lines, and analyzed the effects of this protein on cell migration and metastasis. We demonstrated that ASS1 silencing suppressed cell migration in the MKN45 and 3IB2 cell lines. ASS1 knockdown significantly reduced liver metastasis in mice after the intrasplenic implantation of 3IB2 cancer cell clones. To determine whether arginine restriction may represent a therapeutic approach to treat gastric cancer, the sensitivity of tumor cells to arginine depletion was determined in gastric cancer cells. Arginine depletion significantly inhibited cell migration in the gastric cancer cell line. The silencing of ASS1 expression in MKN45 and 3IB2 gastric cancer cells markedly decreased STAT3 protein expression. In conclusion, our results indicate that the ASS1 protein is required for cell migration in gastric cancer cell lines.
    關聯: Scientific Reports, v.5 , Article ID 9783
    显示于类别:[Dept. of Senior Service and Health Management] Periodical Articles

    文件中的档案:

    档案 描述 大小格式浏览次数
    29698.pdf4348KbAdobe PDF727检视/开启
    index.html0KbHTML2238检视/开启


    在CNU IR中所有的数据项都受到原著作权保护.

    TAIR相关文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback