Identification of 3 '-hydroxygenistein as a potent melanogenesis inhibitor from biotransformation of genistein by recombinant Pichia pastoris

doi:10.1016/j.procbio.2015.06.007

CNU IR > Pharmacy and Science > Dept. of Cosmetic Science and institute of cosmetic science > Periodical Articles > Item 310902800/29681

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Title:	Identification of 3 '-hydroxygenistein as a potent melanogenesis inhibitor from biotransformation of genistein by recombinant Pichia pastoris
Authors:	Ding, Hsiou-Yu Chiang, Chien-Min Tzeng, Wei-Ming Chang, Te-Sheng
Contributors:	化粧品應用與管理系生物科技系
Keywords:	3′-Hydroxygenistein Genistein Melanogenesis Tyrosinase Inhibition Pichia pastoris
Date:	2015-10
Issue Date:	2016-04-19 19:04:25 (UTC+8)
Publisher:	Elsevier Sci Ltd
Abstract:	A product resulting from the biotransformation of genistein by a recombinant Pichia pastoris was isolated and identified as 3'-hydroxygenistein, on the basis of mass, H-1 NMR, and C-13 NMR spectrophotometric analysis. The maximal product concentration and the conversion yield of the biotransformation in a 51 fermenter were 3.5 mg/1 and 14%, respectively. The inhibitory effects of 3'-hydroxygenistein on tyrosinase activity were investigated in vitro using mushroom tyrosinase. The results showed that 3'-hydroxygenistein potently inhibited tyrosinase activity with an IC50 value of 15.9 mu M. Furthermore, the inhibitory effects of 3'-hydroxygenistein on melanogenesis were also investigated in vitro in cultured B16 melanoma cells, and it was shown that 3'-hydroxygenistein dose-dependently inhibited melanogenesis in non-toxic concentrations. In summary, the 3'-hydroxygenistein that was produced from genistein by the recombinant yeast was confirmed as a potent tyrosinase inhibitor and inhibited melanogenesis in B16 cells. (C) 2015 Elsevier Ltd. All rights reserved.
Relation:	Process Biochemistry, v.50 n.10,pp.1614-1617
Appears in Collections:	[Dept. of Biotechnology (including master's program)] Periodical Articles [Dept. of Cosmetic Science and institute of cosmetic science] Periodical Articles

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