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| 標題: | The effect of ferulic acid ethyl ester on leptin-induced proliferation and migration of aortic smooth muscle cells |
| 作者: | Tsai, Yung-Chieh
Lee, Yen-Mei
Hsu, Chih-Hsiung
Leu, Sy-Ying
Chiang, Hsiao-Yen
Yen, Mao-Hsiung
Cheng, Pao-Yun |
| 貢獻者: | 運動管理系 |
| 關鍵字: | Activated protein-kinases
Oxidative stress
In-vivo
Matrix metalloproteinase-2
Cardiovascular-disease
Cdk inhibitors
Adipose-tissue
Plasma leptin
Cyclin D1
Mechanisms |
| 日期: | 2015-08 |
| 上傳時間: | 2016-04-19 19:03:19 (UTC+8) |
| 出版者: | Nature Publishing Group |
| 摘要: | Leptin is a peptide hormone, which has a central role in the regulation of body weight; it also exerts many potentially atherogenic effects. Ferulic acid ethyl ester (FAEE) has been approved for antioxidant properties. The aim of this study was to investigate whether FAEE can inhibit the atherogenic effects of leptin and the possible molecular mechanism of its action. Both of cell proliferation and migration were measured when the aortic smooth muscle cell (A10 cell) treated with leptin and/or FAEE. Phosphorylated p44/42MAPK, cell cycle-regulatory protein (for example, cyclin D1, p21, p27), beta-catenin and matrix metalloproteinase-9 (MMP-9) proteins levels were also measured. Results demonstrated that leptin (10, 100 ng ml(-1)) significantly increased the proliferation of cells and the phosphorylation of p44/42MAPK in A10 cells. The proliferative effect of leptin was significantly reduced by the pretreatment of U0126 (0.5 mu M), a MEK inhibitor, in A10 cells. Meanwhile, leptin significantly increased the protein expression of cyclin D1, p21, beta-catenin and decreased the expression of p27 in A10 cells. In addition, leptin (10 ng ml(-1)) significantly increased the migration of A10 cells and the expression of MMP-9 protein. Above effects of leptin were significantly reduced by the pretreatment of FAEE (1 and 10 mu M) in A10 cells. In conclusion, FAEE exerts multiple effects on leptin-induced cell proliferation and migration, including the inhibition of p44/42MAPK phosphorylation, cell cycle-regulatory proteins and MMP-9, thereby suggesting that FAEE may be a possible therapeutic approach to the inhibition of obese vascular disease. |
| 關聯: | Experimental And Molecular Medicine, v.47, e180 |
| 顯示於類別: | [運動管理系] 期刊論文
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