Genome mining and molecular characterization of the biosynthetic gene cluster of a diterpenic meroterpenoid, 15-deoxyoxalicine B, in Penicillium canescens

doi:10.1039/c5sc01965f

Chia Nan University of Pharmacy & Science Institutional Repository > 藥理學院 > 藥學系(所) > 期刊論文 > Item 310902800/29565

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標題:	Genome mining and molecular characterization of the biosynthetic gene cluster of a diterpenic meroterpenoid, 15-deoxyoxalicine B, in Penicillium canescens
作者:	Yaegashi, Junko Romsdahl, Jillian Chiang, Yi-Ming Wang, Clay C. C.
貢獻者:	藥學系(含碩士班)
關鍵字:	Mycophenolic-acid biosynthesis Aspergillus-fumigatus Acetylcholinesterase Polyketide Inhibitors dDficient Alkaloids Chemistry Mechanism Decaturin
日期:	2015
上傳時間:	2016-04-19 19:00:27 (UTC+8)
出版者:	Royal Soc Chemistry
摘要:	Meroterpenoids are a class of secondary metabolites that are produced from polyketide and terpenoid precursors. 15-Deoxyoxalicine B (1) belongs to one structural group consisting of a unique pyridinyl-alpha-pyrone polyketide subunit and a diterpenoid subunit connected through a characteristic asymmetric spiro carbon atom. An understanding of the genes involved in the biosynthesis of this class of compounds should provide a means to facilitate engineering of second-generation molecules and increasing production of first-generation compounds. We found that the filamentous fungus Penicillium canescens produces 15-deoxyoxalicine B (1). Using targeted gene deletions, we have identified a cluster of 12 responsible contiguous genes. This gene cluster includes one polyketide synthase gene which we have designated olcA. Chemical analysis of wild-type and gene deletion mutant extracts enabled us to isolate and characterize 7 additional metabolites that are either intermediates or shunt products of the biosynthetic pathway. Two of the compounds identified have not been reported previously. Our data have allowed us to propose a biosynthetic pathway for 15-deoxyoxalicine B (1).
關聯:	Chemical Science, v.6 n.11, pp.6537-6544
顯示於類別:	[藥學系(所)] 期刊論文

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