應用酵素連結免疫分析法篩試戴奧辛污染場址之可行性研究

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Title:	應用酵素連結免疫分析法篩試戴奧辛污染場址之可行性研究 The Feasibility on Application of Enzyme-linked Immunosorbent Assay for Screening Dioxin Soil on Contamination Site
Authors:	黃君喆
Contributors:	環境工程與科學系甘其銓
Keywords:	篩試酵素連結免疫分析法戴奧辛校正因子 Screen ELISA Dioxin CAF
Date:	2014
Issue Date:	2015-10-26 20:30:33 (UTC+8)
Abstract:	本研究探討戴奧辛酵素連結免疫分析法(Enzyme-linked Immunosorbent Assay, ELISA)應用於國內戴奧辛高污染場址之案例。研究分兩部份進行，第一部分為ELISA的分析方法應用，包括樣品的乾燥、戴奧辛萃取、淨化及數據解析。第二部分為使用ELISA針對高污染場址進行戴奧辛快速篩試，再輔以高解析氣相層析儀/高解析質譜儀(High resolution gas chromatograph/high resolution mass spectrometer, HRGC/HRMS)進行確認求得校正因子，以便後續污染整治之利用。研究結果顯示，萃取時間在2個小時後，即可達到從土壤樣品中置換出戴奧辛濃度之最大值；在淨化過程中，以甲苯淋洗量20毫升，即可將活性碳管柱中淋洗出戴奧辛濃度最大值。此外，實場應用部分，採樣規劃所得樣品數量為78個樣品，但須以HRGC/HRMS分析其15%的樣品數量，進而求得校正因子(Calibration Adjustment Factor, CAF)。研究結果顯示，經過CAF修正後，9個樣品呈現陽性反應，其中原HRGC/HRMS分析為7個樣品超過法規標準，ELISA分析亦呈陽性；成本估算由原先全數HRGC/HRMS分析改為ELISA分析，費用可省下97.5萬；分析時間亦是省下16天。實驗結果顯示應用ELISA可適當篩試出超標樣品，若運用於大面積污染調查，大量的樣品可先經由此方法找出其污染範圍及趨勢，必要時再將具有代表性的樣品進一步施做HRGC/HRMS分析。ELISA的分析不僅可節省分析費用、縮短時程、快速掌握污染分佈，更可運用於整治工作開始前之調查。 The research is to establish Enzyme-linked immunosorbent assay (ELISA) for contaminant soil and apply on contaminant site of Dioxins. Phase one is to establish the standard operation procedure of ELISA, including drying soil, extraction, clean up and data analysis. Phase two is to apply ELISA on the contaminant site, meanwhile; to match the data with High resolution gas chromatograph/high resolution mass spectrometer (HRGC/HRMS). The result shows that the concentration of Dioxins reaches the maximum in two hour of extraction. The concentration of Dioxins reaches the maximum from carbon column washed by 20 ml of Toluene. Moreover, in the part of application for the site, 12 of 78 samples have been analyzed by HRGC/HRMS for Calibration Adjustment Factor (CAF).After correction of CAF. The results shows that 9 samples analyzed by ELISA screening test appear positive, 7 of 9 samples analyzed by HRGC/HRMS also appear positive. In the part of cost saving, if all samples analyzed by HRGC/HRMS replaced to ELISA screening test, the cost could saved by 975,000 TWD and 16 days of construction period.ELISA screen test can analyze Dioxin sample quickly. A large number of samples screening by ELISA for the investigation in a large contaminant site can understand the distribution and trend of pollutant. If it’s necessary, the representative samples could be analyzed by HRGC/HRMS. ELISA is not only saving money and time but also is a benefit for investigation.
Relation:	只開放學校IP, 開放日期2018-07-09，學年度：103，91頁
Appears in Collections:	[Dept. of Environmental Engineering and Science (including master's program)] Dissertations and Theses

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