銀葉樹水萃取物之生物活性研究與探討

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標題:	銀葉樹水萃取物之生物活性研究與探討 Evaluation of the Bioactivities of Aqueous Extract From Heritiera littoralis
作者:	陳俊曄
貢獻者:	化粧品應用與管理系蔡玫琳呂昆霖
關鍵字:	玻尿酸酶抗氧化銀葉樹抗菌 hyaluronicdase antioxidant Heritiera littoralis antimicrobial
日期:	2014
上傳時間:	2015-10-26 20:30:01 (UTC+8)
摘要:	本研究以銀葉樹(Heritiera littoralis)樹枝、葉子、果實之水萃取物來評估與探討其抗氧化、抗發炎、抗菌及細胞毒性等生物活性。實驗結果顯示，在抗氧化能力方面，總酚與總類黃酮含量均以樹枝萃取物為最高，分別為403.19±8.77 mg GAE/g extract與 219.79±3.63 mg CE/g extract；在DPPH清除率試驗和還原力試驗中，均以樹枝萃取物效果最佳，其EC50分為18.27±0.53及12.59±1.52 ?g/mL；在總抗氧化能力(TEAC)試驗中，以樹枝萃取物效果最佳，其對trolox的當量濃度為3.55±0.07 m mole TE/g extract；在螯合亞鐵離子試驗中，以樹葉萃取物效果最佳，其EC50為2.17±0.05 mg/mL；而在抑制脂質過氧化試驗中，以樹枝萃取物效果最佳，其抑制率達86.81±0.83%；在去氧核醣核酸(DNA)保護試驗中，則以樹葉萃取物保護DNA效果最佳。在玻尿酸?譜法試驗中，三種樣品對於玻尿酸?皆有顯著抑制之效果；抗菌試驗中，樹枝萃取物對於枯草桿菌具有最佳抑制效果，抑菌圈 15.62±0.63mm。在人類纖維母細胞存活率試驗中，細胞經處理48小時皆無產生細胞毒性。綜合以上結果得知銀葉樹之樹枝水萃取物具有最佳抗氧化、抑制玻尿酸?及抗菌效果，樹葉萃取物則對於螯合金屬離子及保護DNA具有較佳的效果。 The aim of this study was to evaluate the aqueous extracts of Heritiera littoralis branches, leaves, and fruits by Antioxidant, anti-inflammatory, anti-bacterial and cytotoxic assay.Our results showed that total phenolic and flavonoid content of branch extracts were equal to 403.19 ± 8.77 mg GAE/g extract and 219.79 ± 3.63 mg CE/g extract, respectively Branches displayed the lowest value of EC50 (18.27 ± 0.5 and 12.59 ± 1.52 μg/mL) on scavenging the DPPH radicals and reducing power assay. on the TEAC value of branch extracts, its equivalent concentration of trolox was 3.55 ± 0.07 mmole TE / g extract. For the ferrous ion-chelating ability, the lowest EC50 value was exhibited on leaves extracts (2.17±0.05 mg/mL).The best value of inhibiting lipid peroxidation was 86.81±0.83% in branch extracts. On the protective test of DNA, the leaves extracts had the best effect. For the anti-inflammatory test, extracts of branches, leaves, and fruits could inhibit the hyaluronidase activity. For the anti-microbial activity, the branch extract had the good ability to against Bacillus subtilis with the inhibitory zone of 15.62 ± 0.63 mm. For the assay of Hs68 cell viability, these extracts displayed non-cytotoxic effect after 48 hours treatment.Based on the above results that the aqueous extracts of branches from silver leaf tree has the best antioxidant, inhibiting HAase activity and antibacterial effect, then extracts of the leaves had a better capacity on the chelating metal ions and DNA protection.
關聯:	網際網路公開：2019-08-15，學年度：102，75頁
显示于类别:	[化妝品應用與管理系(所)] 博碩士論文

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