黑豆萃取物及金雀異黃酮對 Epstein-Barr 病毒溶裂循環的影響

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Title:	黑豆萃取物及金雀異黃酮對 Epstein-Barr 病毒溶裂循環的影響 Effect of extract from black soybean (Glycine max L.) and genistein on Epstein-Barr virus lytic cycle
Authors:	林雅婷
Contributors:	生物科技系林美惠林翠品
Keywords:	Epstein-Barr 病毒 (EB 病毒)、黑豆萃取物、金雀異黃酮 Epstein-Barr virus (EBV)﹐black soybean extract﹐genistein
Date:	2014
Issue Date:	2015-10-26 20:29:01 (UTC+8)
Abstract:	文獻指出，金雀異黃酮能抑制單純性?疹病毒的 DNA 複製，也影響 Anti-IgG 所誘導的 Epstein-Barr 病毒 (EB 病毒) 再活化。所以本研究將探討黑豆萃取物及金雀異黃酮對溶裂循環誘導劑-丁酸鈉 (SB) 所誘導 EB 病毒溶裂循環的影響。以逆相式高效液相層析分析黑豆萃取物中含有 5% 之金雀異黃酮成分。以 MTT 方法測定黑豆萃取物及金雀異黃酮對含有 EB 病毒的 P3HR1 細胞存活率的影響；以西方墨點法 (Western blotting) 測定 Rta、 Zta、 EA-D EB 病毒溶裂蛋白的表現量；以即時定量聚合?酵素連鎖反應 (Real-time quantitative polymerase chain reaction，Q-PCR) 方法測定黑豆萃取物及金雀異黃酮對 EB 病毒溶裂循環相關基因 BZLF1 及 BRLF1 mRNA 的表現及 DNA 的複製，結果顯示黑豆萃取物及金雀異黃酮在不具細胞毒性之濃度下，抑制溶裂循環誘導劑-丁酸鈉 (SB) 所誘導之 EB 病毒極早期基因 BZLF1 及 BRLF1 的轉錄，而導致溶裂蛋白 Rta、 Zta 及 EA-D 表現量下降，進而影響 EB 病毒 DNA 的複製；此結果說明黑豆萃取物及金雀異黃酮具有抑制 EB 病毒溶裂循環的活性。 Previous studies showed that genistein inhibites DNA replication of herpes simplex virus and also affects anti-IgG induced Epstein-Barr virus (EBV) reactivation. Therefore, this study investigates the effects of black soybean extract and genistein on the EBV lytic cycle induced by sodium butyrate (SB). Reverse-phase high-performance liquid chromatography analysis of black soybean extract, shows that black soybean extract contained 5% genistein. MTT assay was performed to determine the effect of black soybean extract and genistein on viability of P3HR1 cells. Western blot analysis was used to measure the expression of Rta, Zta, EA-D EBV lytic proteins. Real-time quantitative polymerase chain reaction (Q-PCR) method was used for the determination of the EBV lytic related genes, BZLF1 and BRLF1 mRNA expressions and EBV DNA copy number. My study finds that black soybean extract and genistein in non-cytotoxic concentrations, inhibit the transcription of the BZLF1 and BRLF1 genes that is activated by SB, and the expression of lytic proteins, Rta, Zta and EA-D expression, thereby affecting the EBV DNA replication. These results indicate that black soybean extract and genistein inhibit the EBV lytic cycle.
Relation:	網際網路公開：2019-12-31，學年度：102，69頁
Appears in Collections:	[Dept. of Biotechnology (including master's program)] Dissertations and Theses

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