| Abstract: | 生長於亞熱帶地區的藥用香草植物左手香 (Pogostemon cablin ) 、歐洲地區的百里香植物 ( Thymus vulgaris ) ，皆含有香芹酚 ( Carvacrol ) 及百里香酚 ( Thymol ) 等成份，此兩種化合物是為同分異構物( Isomer )的單?酚類，且具有抗發炎、抗氧化、驅蚊等功效。在本研究中，將比較香芹酚與百里香酚在左手香及百里香中的抗氧化、抗菌性以及忌避性的效用。實驗方法是利用薄層層析（ Thin Layer Chromatography，TLC ）、氣相層析質譜儀 ( Gas Chromatography-Mass Spectrophotometer，GC-MS )，進行定性及定量分析，接著利用 DPPH 自由基清除法做抗氧化試驗，以大腸桿菌及金黃色葡萄球菌進行抗菌相關等試驗，及左手香、百里香粗萃物對於埃及斑蚊的忌避效果。新鮮左手香葉、新鮮百里香葉及市售乾燥百里香葉乙醇萃取物中，香芹酚含量分別是2.05%、0.00%及3.41%；百里香酚含量分別是0.00%、0.52%及0.93%。抗氧化 DPPH 自由基清除結果發現香芹酚IC50為77.63 mg/mL、百里香酚IC50為 147.50 mg/mL、左手香乙醇萃取物 IC50為 4.329 mg/mL、百里香乙醇萃取物 IC50為 1.912 mg/mL，百里香乙醇萃取物抗氧化能力較佳；在抑菌試驗結果百里香酚有較佳之抑菌效果；忌避測試評估方面，標準品香芹酚有長達四個小時的有效保護時間，而在真實樣品中則是以左手香乙醇萃取物較佳。
Grown in subtropical regions of the medicinal herb plants left incense ( Pogostemon cablin ), thyme plants Europe ( Thymus vulgaris ), both containing carvacrol and thymol and other ingredients, these two compounds is isomers monoterpene phenols , and has anti-inflammatory, antioxidant, insect repellent and other effects.In the present study, carvacrol and thymol comparing the antioxidant in the Pogostemon cablin and Thymus vulgaris, and the antimicrobial effectiveness of the repellent . Experimental Method using thin layer chromatography (Thin Layer Chromatography, TLC), gas chromatography mass spectrometer (Gas Chromatography-Mass Spectrophotometer, GC-MS), qualitative and quantitative analysis, and then make use of DPPH radical scavenging antioxidant law test, E. coli and Staphylococcus aureus antibacterial test, and left fragrant thyme crude extract for Aedes aegypti repellent effect.The results showed that the left hand fresh bay leaves, fresh thyme leaves and dried thyme leaves commercial ethanol extract , carvacrol contents were 2.05 %, 0.00 % and 3.41% ; thymol contents were 0.00% , 0.52 % and 0.93% . DPPH radical scavenging antioxidant found that carvacrol IC50 was 77.63 mg / mL, thymol IC50 was 147.50 mg / mL, left Hong ethanol extract IC50 of 4.329 mg / mL, the ethanol extract of thyme IC50 of 1.912 mg / mL, thyme ethanol extract antioxidant capacity better ; thymol test results in inhibition had a better antibacterial effect ; repellent test evaluation, standard carvacrol have effective protection for up to four hours of time , but in the real sample it is better left Hong ethanol extracts. |
