作為藥物輸送系統來提高藥物的治療功效形式相當多種，而近年來高分子奈米微胞 (polymeric micelle) 已被越來越多的研究指出，能夠作為水難溶解性藥物的輸送載體，而一般常見的高分子奈米微胞通常由兩性共聚物 (amphiphilic copolymer) 構成。本研究目的在於設計兩性共聚物高分子形成奈米微胞，疏水端的部分能包覆藥物形成核心，而以樹枝狀聚合物設計的親水端也能供藥物的裝載，使其具備更高的載藥量與穩定性，由於此藥物載體具有兩種不同高分子材料，因此也會有兩種不同的藥物釋放速率。本研究利用生物可分解材料 poly(lactide-co-glycolide)(PLGA) 與不同世代 (generation) 之樹枝狀聚胺基甲酸酯 (G 2.0 PUAD、G 3.0 PUAD) 以脫水合成的方式，形成兩性共聚物。利用傅立葉紅外線光譜 (FT-IR) 與核磁共振光譜儀 (NMR) 確定其化學結構。並進行以下之性質研究：DLS與TEM粒徑分析、細胞毒性、藥物溶解度、藥物負載及藥物釋控。 Drug delivery system to improve the efficacy of treatment have a considerable types. In recent years some studies indicate that polymer nano-micelles can be used as delivery vehicles for drug with lower solubility. Polymeric micelles usually consists of amphiphilic copolymer. The purpose of this study is to design amphiphilic copolymer to form polymeric micelles. Hydrophobic side of the copolymer form the core, and then dendrimer hydrophilic end stabilize the particles in the aqueous medium. The amphiphilic copolymer was obtained through the reaction between the PLGA and different generation polyurethane dendrimers (PUAD) via dehydration. The chemical structure of the amphiphilic copolymer was indentified by fourier transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance spectroscopy (NMR). We also studied various properties about the synthesized amphiphilic copolymer as DLS and TEM to determine particle size, cytotoxicity, the increasement of drug solubility, drug loading, and controlled release.
