草坪為人類生活帶來許多益處，本研究所要鑑別的兩種地毯草Axonopus compressus(熱帶地毯草)和Axonopus affinis (巴西地毯草)已經引進台灣多年，所分布的地區與氣候條件相近，在某些環境條件下(如：乾燥、蔭影)，A. compressus也會長出相對比較長的窄葉，和A. affinis在外觀上沒有什麼不同，以致發生爭議。ISSR(inter-simple-sequence-repeat)分子標誌技術具有許多優點，利用真核生物基因體中，普遍存在許多簡單重複序列(SSR/simple sequence repeat)的原理，以SSR序列作為引子，擴增出兩段SSR之間的區域序?，是為ISSR。擴增出來的序列長度具有多型性，可以快速推導出物種間親緣關係，可作為物種的生物分子辨識標誌。本研究以10 個ISSR引子進行PCR來鑑別兩個疑似熱帶地毯草和巴西地毯草的待鑑定樣本。實驗結果顯示UBC812、UBC825、UBC827、UBC841、UBC850、UBC889等6個引子可有效鑑別兩個待測的地毯草品種。本研究結果以分子標記取代傳統鑑定，可區分外觀極易混淆之地毯草品種，於商業採買糾紛中可提供有力的分子證據。 Axonopus compressus (Swarty) Beauv. and Axonopus affinis Chase have been introduced to Taiwan for many years. The climatic conditions and distribution regions of these two carpet grasses are similar. A. compressus in some circumstances, such as dry, shady conditions, produces long comparatively narrow leaves, little different superficially from A. affinis. ISSR molecular marker is effective and convenient way to identify species. It is the use of simple sequence repeats(SSR) as a primer prevalent in eukaryotic genomes amplifying DNA fragment polymorphism for assessing Phylogenetic relationships among species. This study applies 10 ISSR primers to identify two suspected carpet grasses species( Axonopus compressus. and Axonopus affinis.)The experimental results show that primers UBC812、UBC825、UBC827、UBC841、UBC850 and UBC889 can identify two tested carpet grasses effectively. The results of this study prove that the ISSR method can identify the easily confused species, and therefore the method of traditional identification can be replaced by the ISSR method. Consequently, the ISSR method provides a better way to solve the problems of commercial dispute due to species identification.
