English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 18034/20233 (89%)
造訪人次 : 23343264      線上人數 : 526
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 查詢小技巧:
  • 您可在西文檢索詞彙前後加上"雙引號",以獲取較精準的檢索結果
  • 若欲以作者姓名搜尋,建議至進階搜尋限定作者欄位,可獲得較完整資料
  • 進階搜尋
    請使用永久網址來引用或連結此文件: https://ir.cnu.edu.tw/handle/310902800/29000


    標題: 探討牛樟芝萃取物對藥物誘導人類肝癌細胞死亡之影響
    To investigate the effect of Antrodia cinnamomea extracts on the anticancer activity of drugs treatment in hepatocellular carcinoma cells
    作者: 吳韋德
    貢獻者: 生物科技系
    洪瑞祥
    關鍵字: Huh-7
    EAC
    藥物
    牛樟芝
    肝癌
    HepG2
    EAC
    Drugs
    Antrodia cinnamomea
    Hepatocellular carcinoma
    Huh-7
    HepG2
    日期: 2015
    上傳時間: 2015-10-21 17:08:00 (UTC+8)
    摘要: 肝癌在全球為排名第三的惡性腫瘤,超過75% 的肝癌病患出現在亞太地區。在台灣每年約有3700位病人因肝癌而死亡。目前有許多的化學物藥物已經使用在 肝癌的治療上。牛樟芝為醫藥用真菌且在台灣被當作保健食品,先前文獻指出牛樟芝萃取物可以抑制腫瘤細胞生長、維護肝臟功能、抗發炎及抗氧化等功能。然而牛樟芝對於肝癌患者在接受藥物治療的安全評估不清楚。因此牛樟芝萃取物對藥物誘導肝癌細胞死亡的影響需要去確認。本研究想要去探討牛樟芝萃取物對於藥物處理肝癌細胞時所扮演的角色和機制影響。首先我們以酒精來萃取牛樟芝培養基 (EAC),初步結果顯示當給予高濃度的藥物 (5-25 M) 或牛樟芝萃取物 (200-250 g/ml) 處理48小時後可以明顯抑制Huh-7和HepG2肝癌細胞生長,而當以藥物合併EAC處理時發現EAC可以明顯增強Huh-7和HepG2細胞對低濃度藥物的敏感度。而在細胞的外觀上,藥物合併EAC處理時可以使Huh-7細胞從平貼變成圓形。這初步結果顯示EAC對於藥物在處理肝癌細胞時扮演重要角色。因此在本論文中我們進一步分析藥物合併EAC對Huh-7細胞週期的影響,G2 / M期細胞的百分比顯著增加;再更進一步分析Hun-7細胞週期相關蛋白質,經合併處理有抑制現象。在動物模式上,藥物合併EAC明顯抑制腫瘤生長。這樣的結果可以提供一個重要的牛樟芝保健食品安全資訊給在以藥物治療肝癌的病人。
    Hepatocellular carcinoma (HCC) is the third most common malignancy worldwide. More than 75% of HCC cases occur in the Asia-Pacific region. In Taiwan, there are about 3,700 patients a year die from liver cancer. There are many chemical drugs have been used in liver cancer therapy. Antrodia cinnamomea is a medicinal fungus that has been served as a functional food in Taiwan for decades. Previous studies have shown that the secondary metabolites form Antrodia cinnamomea have various activities such as antitumor, protection of liver function, anti-inflammation and antioxidant. However, the safety and the possible interaction of Antrodia cinnamomea extracts with drugs in liver cancer patients have been not studied yet.In the current project, we want to identify the possible role and mechanisms of Antrodia cinnamomea extracts in modulating the activity of drugs in hepatoma cells both in vitro and in vivo. First, Antrodia cinnamomea extracts (EAC) were isolated from culture medium using ethanol. Our preliminary results indicated that the exposure to high concentrations of drugs (5-25 M) or Antrodia cinnamomea extracts (200-250 g/ml) for 48 h caused significant death in Huh-7 and HepG2 cells. In addition, EAC could sensitive tumor cells to low dose of drugs in Huh-7 and HepG2 cells.Considering the cell morphology, the combination of drugs with EAC caused progressive changes in Huh-7 cells from flat to round. Therefore, our preliminary results indicated that EAC could have a positive role in the application of drugs for the treated hepatocellular carcinoma. In this paper, we will further analyze the effect of combination on cell cycle for Huh-7 cells. The percentage of G2/M phase cells notably increased. Then further analysis of cell cycle related proteins on Hun-7 cells, the combination-treated has inhibition. In animal models, the combination significantly inhibition of tumor growth. These results could provide important insight in chemotherapy and safety of Antrodia cinnamomea.
    關聯: 網際網路公開:2020-12-31,學年度:103,120頁
    顯示於類別:[生物科技系(所)] 博碩士論文

    文件中的檔案:

    檔案 描述 大小格式瀏覽次數
    index.html0KbHTML1252檢視/開啟


    在CNU IR中所有的資料項目都受到原著作權保護.

    TAIR相關文章

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋