耐鋅/銅嗜油菌之分離與生理特性探討及其於石油碳氫化合物污染處理

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Title:	耐鋅/銅嗜油菌之分離與生理特性探討及其於石油碳氫化合物污染處理 Isolation and Physiological Characteristics of Zn/Cu-Tolerant Oil-Degrading Bacteria and the Performance for Treatment of Petroleum-Hydrocarbon Contamination
Authors:	Jumpada Yingcharoen
Contributors:	環境工程與科學系張家源劉瑞美
Keywords:	鋅/銅耐受性石油碳氫汙染嗜油菌 Zn/Cu-tolerance petroleum-hydrocarbon contamination oil-degrading microbes
Date:	2015
Issue Date:	2015-10-21 17:07:17 (UTC+8)
Abstract:	本研究由受油品與鋅/銅汙染之油泥中篩選分離兼具有效降解柴油或甲苯與耐受鋅/銅的高效降解菌，研究中共計篩選出10株高效降解菌，其中包括：甲苯降解菌T5, T6, T7, T10, T11與T12；柴油降解菌D2, D5, D6與D7，進一步利用16S rDNA鑑定各菌種，結果發現主要之菌種為Achromobacter insolitus (D2), Candida spp. (D5), Janthobacter polyaromaticivorans (D6與D12), Ochrobactrum intermedium (D7), Streptomyces caelestis (T1與T5), Gordonia amicalis (T6), Brevibacillus brevis (T10), Bacillus fumarioli (T11)與真菌(T7)。運用氣相層析火焰離子化偵檢器(gas chromatography/flame ionization detector, 簡稱GC/FID)測定高效降解菌對柴油或甲苯之降解能力，比較各菌株之降解力，結果可知T6, T7與T11具有較佳之甲苯降解能力；D2, D5與 D6則具有較佳之柴油降解能力。進一步探討各降解菌的重金屬耐受性與共代謝能力，本研究篩選之甲苯分解菌均能忍受250 ppm Zn，柴油分解菌D2, D5與D7能忍受250 ppm Zn，高Zn濃度則對多數菌種產生抑制作用，其中T7與D5可忍受高濃度(500 ppm)；菌種之生長較易受銅影響，篩選出之菌種中僅以T7與D5能耐受高濃度Cu，後續利用化學連續萃取法分析探討耐受菌種之重金屬分布型態，以探討重金屬在細胞中之累積機制。結果顯示，細胞外之吸附量(biosorption)為59.7~70.8%，細胞內累積量(bioaccumulation)則約在29.2~40.3 %，微生物對重金屬之耐受主要是將重金屬排除於細胞外，或將重金屬結合於細胞壁與細胞外聚合物。 The experiments were performed to screen and isolate the bacteria that might be suitable for bioremediation purposes to remove toluene or diesel from polluted soil in presence of Zn and Cu. Ten bacterial strains were isolated from different sources of soil slurry in Taiwan, including 6 strains of toluene-degrading bacteria (T5, T6, T7, T10, T11 and T12) and 4 strains of diesel oil-degrading bacteria (D2, D5, D6 and D7). To illustrate the result of 16S rDNA approach that the dominate bacteria are Achromobacter insolitus (D2)， Candida spp. (D5)，Xanthobacter polyaromaticivorans (D6 and T12)，Ochrobactrum intermedium (D7)，Streptomyces caelestis (T1 and T5)，Gordonia amicalis (T6)，Brevibacillus brevis (T10)，Bacillus fumarioli (T11) and Fungus (T7). Their ability to degrade toluene and diesel were analyzed by a gas chromatograph coupled to a FID detector (GC-FID) method. The observations indicated that T6, T7, T11 and D2, D5, D6 have the high performance on toluene and diesel degradation respectively. In the research, tolerant to heavy metal and the effect of different nutrients on co-metabolism system was studied. Whether show the inhibition to the growth or increase efficiency to the percent removal of the petroleum-hydrocarbon. The results showed, 250 ppm Zn-tolerant on all toluene-strains and D2, D5, D7 strains while 250 ppm Cu-tolerant revealed to T7 strain. The strains was evaluated the accumulation of heavy metal on cell mechanism by sequential chemical extraction (SCE), thus, resulting 59.7-70.8 % biosorption and 29.2-40.3 % bioaccumulation.
Relation:	網際網路公開：2016-07-28，學年度：103，129頁
Appears in Collections:	[Dept. of Environmental Engineering and Science (including master's program)] Dissertations and Theses

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