本研究主要藉由奈米結構脂質載體具有晶格缺陷之結構，進行包覆蝦紅素，比較使用不同界面活性劑Tween? 80 (Polysorbate 80)、Poloxamer? 188(Pluronic? F-68)及Plantacare? 810 (Caprylyl/capryl glucoside)，並添加不同濃度之維生素E，其目的為避免活性成分氧化及降解。利用高壓均質乳化法 (500 bar / 5 cycles) 進行奈米結構脂質載體之製備，並儲存於4℃、25℃及45℃進行奈米結構脂質載體粒徑大小、界面電位、包覆率、安定性、熱差分析、型態分析及皮膚有效性之物性評估。 由實驗結果顯示，使用不同界面活性劑經高壓均質法製備奈米結構脂質載體之平均粒徑範圍在200 nm以內，所有配方之平均PdI值皆小於0.2，平均界面電位值在 -30 mV以上，且包覆率為100%。經由90天安定性評估得知，相較於界面活性劑Tween 80及Poloxamer 188，使用界面活性劑Plantacare? 810製備NLCs包覆蝦紅素其總量回收率皆在90%以上，能有效緩解包覆物質洩漏及降解之情形產生，不需額外添加維生素E防止蝦紅素氧化。 Astaxanthin encapsulated with nanostructured lipid carriers (NLCs) was investigated in this study. Three different surfactants including Tween? 80 (Polysorbate 80), Poloxamer? 188 (Pluronic? F-68), Plantacare? 810 (Caprylyl / capryl glucoside) and vitamin E were used to evaluate the stability of NLCs. The purpose of the present research was to avoid oxidation and degradation of the active ingredient. The nanostructured lipid carriers were prepared by high pressure homogenizers at 500 bar 5 cycles. The stable and physical property investigation of Astaxanthin loaded nanostructured lipid carriers were stored at 4, 25 and 45℃. The experimental results show that nanostructured lipid carriers diameter were around 200 nm with a narrow polydispersity index (PdI) lower than 0.2, the mean value of zeta potential were more than -30 mV. The encapsulation efficiency of all formulations were 100%. The stability test results showed the surfactant of Plantacare? 810 is better than Tween? 80 and Poloxamer? 188. The total NLCs recovery results of Plantacare? 810 were more than 90% after 90 days. The surfactant of Plantacare? 810 is can effectively reduce leak and degradation of active ingredient, and don’t add vitamin E to prevent oxidation of Astaxanthin.
