| 摘要: | 現今社會中安全無毒已成為一種新的趨勢，消費者對於尋求添加功效性天然物之化粧品需求也與日俱增。在過去食品及醫學領域等研究中，有許多關於乳酸菌醱酵物的功能性研究，但對於化粧品的領域相對較少，因此本研究將不同中草藥萃取物與乳酸菌（Lactobacillus plantarum, BCRC 10069）進行醱酵反應，醱酵後所得到的醱酵物樣品（編號分別為LABF01～11），進行3T3纖維母細胞毒性分析、清除自由基試驗、基質金屬蛋白?（matrix metalloproteinases, MMPs）活性抑制分析、抗過敏活性分析、美白活性分析及抑菌能力試驗等化粧品功能性評估。實驗結果顯示（1）在3T3纖維母細胞毒性試驗中，3種乳酸菌醱酵物（LABF01、02及06）處理細胞48小時內，其存活率大於80%的安全劑量皆小於5.0%；（2）11種乳酸菌醱酵物對於自由基（DPPH）均無明顯的清除作用；（3）7種乳酸菌醱酵物（LABF03、04、05、07、08、09及11）在2.5～10.0%的濃度下，反應24小時，對MMP-2和MMP-9具有顯著的抑制作用；（4）4種乳酸菌醱酵物（LABF03、04、05及08）在1.0～10.0%的濃度下，處理24小時，對玻尿酸?（hyalurondiase）具有顯著的抑制作用；（5）5種乳酸菌醱酵物（LABF03、04、05、08及09）對於大腸桿菌、金黃色葡萄球菌及綠膿桿菌皆具顯著的抑菌作用，抑菌圈直徑介於16.3~29.0 mm；（6）在酪胺酸?活性抑制分析中，5種乳酸菌醱酵物（LABF04、05、08、09和11）對酪胺酸?活性具顯著的抑制作用。綜合上述實驗結果，乳酸菌醱酵物本身除了能應用於開發抗老化、抗過敏及美白的功能性化粧品外，其抑菌能力還可以降低化粧品中防腐劑的使用量。因此，乳酸菌醱酵物不僅能夠應用於醫學及保健食品領域，未來在化粧品功能性領域的應用極具潛力。
Safety and non-toxicity are the new trend in modern society. The demand to find effective natural cosmetic is increasing day by day for consumers. There are many functional studies on the lactic acid bacteria fermented (LABF) products in previous studies of food industry and medical field, but relatively few in the cosmetic field. Therefore, we use different herbal extracts and lactic acid bacteria (Lactobacillus plantarum, BCRC 10069) to carry out fermentation in this study. After fermentation the resulting products (NO. LABF01～11) were analyzed for cosmetic functional assessment about MTT assay, anti-allergy assay, anti-tyrosinase assay, anti-MMPs assay, antioxidation assay, and anti-bacterial assay. The results presented here showed that: (1) in MTT assay 3T3 cells were treated with 3 LABF samples (LABF01、02及06) respectively in 48 hours, safe doses for cell survival rates more than 80% were less than 5.0%; (2) DPPH radical scavenging activities of 11 LABF samples were not obvious; (3) 7 LABF samples (NO. LABF03, 04, 05, 07, 08, 09 and 11) exhibited marked inhibition on MMP-2 and MMP-9 at the concertration of 2.5～10.0% in 24 hours; (4) in anti-allergy assay 4 LABF samples (NO. LABF03, 04, 05 and 08) significantly suppressed hyalurondase activities at the concentration of 1.0～10.0% in 24 hours; (5) 5 LABF samples (NO. LABF03, 04, 05, 08 and 09) displayed a powerful antibacterial effect on Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa, and diameters of inhibition zone were between 16.3~29.0 mm; (6) 5 LABF samples demonstrated potent inhibitory activities (NO. LABF04, 05, 08, 09 and 11) against mushroom tyrosinase. According to above experimental results, in addition to the anti-aging, anti-allergy, and whitening functions to cosmetic applications, the strong anti-bacterial activities of LABFs also could reduce the amount of preservatives used in cosmetics. In conclusion, lactic acid bacteria fermented products can not only be applied to the field of medicine and health food, but also can be employed to functional areas for cosmetics extensively. |
