比較不同萃取法所得之金銀花萃取物於生物活性上之差異

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Title:	比較不同萃取法所得之金銀花萃取物於生物活性上之差異 The differences in the biological activities of Lonicera japonica Thunb. by different extraction methods
Authors:	呂芷縈
Contributors:	化粧品應用與管理系蔡玫琳
Keywords:	痤瘡桿菌脂解酶抗發炎金銀花 Lipase Anti-inflammatory Lonicera japonica P. acnes
Date:	2015
Issue Date:	2015-10-21 17:04:40 (UTC+8)
Abstract:	痤瘡桿菌為革蘭氏陽性菌，通常存在皮膚較深層毛囊（Hair follicle）內，為人體皮膚共生菌，被認為是在發炎病變之發病機制中發揮重要作用。因此，抑制痤瘡桿菌為治療炎症性痤瘡之主要目標之一。　　本研究將金銀花（Lonicera japonica）之不同部位以水溶液萃取法或50%乙醇萃取法並經減壓濃縮、冷凍乾燥後獲得萃取物，參照痤瘡之發炎機轉，進行抗發炎、抗氧化、抗菌及抑制脂解?活性等試驗。實驗結果顯示金銀花對DPPH及β-Carotene之抑制能力以乙醇萃取之葉片具有較強活性，於抑制5-Lipoxygenase（5-LOX）能力之活性更是高於標準品α-Bisabolol。　　抗菌能力評估，顯示金銀花對痤瘡桿菌具有良好抑菌效果，乙醇萃取金銀花之花朵及莖部於低濃度時呈現抑菌活性，乙醇萃取金銀花之花朵，更在濃度20mg/mL時達到殺菌效果。而萃取物於抑制痤瘡桿菌脂肪?之能力，以乙醇萃取金銀花之葉片效果最佳，其IC50值為4.82±0.57 mg/mL。另外，抑制念珠菌脂肪?之能力，以乙醇萃取金銀花之花朵效果最好，於濃度4.76±0.41mg/mL時可達IC50。　　此外，發現金銀花之萃取物具有酪胺酸?活性增強之效果。且金銀花之萃取物於波長320-400nm（UVA）及280-320nm（UVB）有良好之吸收值，由上述顯示，金銀花具有良好之抗發炎能力，更證實金銀花之多元化功效，於化妝品之應用上具有良好潛力。 Propionibacterium acnes (P. acnes), a Gram-positive bacterium, colonizes the duct of sebaceous follicle and known as a skin commensal organism. In recent years, P. acnes is recognized as a pathogen of acne vulgaris and plays an important role in inflammatory acne lesion pathogenesis. Therefore, to treat inflammatory acnes, one of our targets is to inhibit P. acnes. In this research, the aqueous and ethanol extracts of different parts of Lonicera japonica were concentrated and freeze dried to give residues. We discovered that compared with other parts of L. japonica, the ethanol extract of blades was more effective to inhibit DPPH, beta-carotene, and 5-lipoxygenase than its standard alpha-bisabolol, especially.We also noticed that L. japonica was effective to inhibit the growth of P. acnes. Ethanol extracts of flowers and stalks were bacteriostatic in low concentration, and extract of flowers was disinfectant at 20 mg/ml. Besides, ethanol extract of blades was the best inhibitor to P. acnes lipase when IC50 was 4.82?0.57 mg/ml, and ethanol extract of flowers was the best inhibitor to Candida rugosa lipase when IC50 was 4.76?0.41 mg/ml. Finally, extract of L. japonica elevated tyrosinase activity and had appropriate absorption value at a wavelength of 320-400 nm (UVA) and 280-320 nm (UVB).In conclusion, from previous results we confirmed the ability of L. japonica to anti-inflammation, its multi-function, and potential in application of cosmetics.
Relation:	網際網路公開：2020-08-31，學年度：103，98頁
Appears in Collections:	[Dept. of Cosmetic Science and institute of cosmetic science] Dissertations and Theses

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