生育三烯醇是維他命E家族中的成員之一，以天然化合物的形式存在少數植物種子中，如小麥、堅果。研究指出生育三烯醇具有多元的生物活性，包括保護神經、抗癌、抗發炎及降低膽固醇等。本研究目的是探討 α-、γ-及δ-生育三烯醇 (αT3、γT3及δT3) 對於 3T3-L1脂肪細胞之抗增生及脂肪堆積效應的影響，並對 3T3-L1 脂肪細胞的能量及脂肪生合成作用分子機制進行研究。結果顯示，30 μM γT3 能最有效抑制3T3-L1細胞增生作用。另外，γT3 可以顯著降低 3T3-L1 脂肪細胞之脂肪堆積效應 (P<0.05)。西方墨點分析結果發現，γT3可升調節 CD36、Sirt (1及3)、p-AMPK及 LXRα蛋白質表現，並降調節 ACC、PPAR-γ 及 FAS 的表現。本研究結果發現，γT3 呈現抗脂肪細胞增生及抗脂肪生合成作用，其可能是藉由透過 Sirt1、p-AMPK、FAS 及 PPAR-γ 訊息途徑所致。 Tocotrienols, members of the vitamin E family, are natural compounds only found in certain numbers of plant seeds; for example, wheat and nuts. Tocotrienols exhibit biological activities such as neuroprotective, anti-cancer, anti-inflammatory and cholesterol lowering properties. In this study, our aims were to examine the anti-proliferative and lipid accumulation effects of α-, γ- and δ-tocotrienols (αT3, γT3 and δT3) in 3T3-L1 adipocytes, and to study the energy and adipogenesis molecular mechanism (s) of action in 3T3-L1 cells. The results displayed that 30 μM γT3 was effective in inhibiting the cell proliferation. In addition, γT3 significantly reduced the lipid accumulation effect in 3T3-L1 adipocytes. Western blot data showed that γT3 treatment up-regulated the expression of CD36, Sirt (1and 3), p-AMPK and LXRα proteins, but it down-regulated the expression of ACC, PPAR-γ and FAS. These results have demonstrated that γT3 possesses anti-proliferative and anti-adipogenic effects; its molecular mechanism of action could be through the modulation of the Sirt1, LXRα, p-AMPK and PPAR-γ signaling pathways.
