Therapeutic ROS targeting of GADD45 gamma in the induction of G2/M arrest in primary human colorectal cancer cell lines by cucurbitacin E

doi:10.1038/cddis.2014.151

Chia Nan University of Pharmacy & Science Institutional Repository > 環境永續暨休閒學院 > 運動管理系 > 期刊論文 > Item 310902800/28720

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://ir.cnu.edu.tw/handle/310902800/28720

標題:	Therapeutic ROS targeting of GADD45 gamma in the induction of G2/M arrest in primary human colorectal cancer cell lines by cucurbitacin E
作者:	Hsu, Y-C Huang, T-Y Chen, M-J
貢獻者:	運動管理系
關鍵字:	Cucurbitacin E Colorectal cancer (CRC) G2/M arrest Growth arrest and DNA damage 45 (GADD45)
日期:	2014-04
上傳時間:	2015-05-06 21:26:23 (UTC+8)
出版者:	Nature Publishing Group
摘要:	Cucurbitacin E (CuE) or alpha-elaterin is a natural compound previously shown to be an antifeedant as well as a potent chemopreventive agent against several types of cancer. The present study investigated the anticancer effects of CuE on colorectal cancer (CRC) using primary cell lines isolated from five CRC patients in Taiwan, Specifically, we explored the anti-proliferation and cell cycle G2/M arrest induced by CuE in CRC cells. MPM-2 flow cytometry tests show that CuE-treated cells accumulated in metaphase (CuE 2.5-7.5 mu M). Results further indicate that CuE produced G(2)/M arrest as well as the downregulation of CDC2 and cyclin B1 expression and dissociation. Both effects increased proportionally with the dose of CuE; however, the inhibition of proliferation, arrest of mitosis, production of reactive oxygen species (ROS), and loss of mitochondrial membrane potential (Delta Psi m) were found to be dependent on the quantity of CuE used to treat the cancer cells. In addition, cell cycle arrest in treated cells coincided with the activation of the gene GADD45(alpha, beta, gamma). Incubation with CuE resulted in the binding of GADD45 gamma to CDC2, which suggests that the delay in CuE-induced mitosis is regulated by the overexpression of GADD45 gamma. Our findings suggest that, in addition to the known effects on cancer prevention, CuE may have antitumor activities in established CRC.
關聯:	Cell Death & Disease, v.5, e1198
显示于类别:	[運動管理系] 期刊論文

文件中的档案:

档案	描述	大小	格式	浏览次数
28720.pdf		2237Kb	Adobe PDF	523	检视/开启
index.html		0Kb	HTML	1717	检视/开启

在CNU IR中所有的数据项都受到原著作权保护.

TAIR相关文章

数据加载中.....