alpha-Solanine Inhibits Invasion of Human Prostate Cancer Cell by Suppressing Epithelial-Mesenchymal Transition and MMPs Expression

doi:10.3390/molecules190811896

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標題:	alpha-Solanine Inhibits Invasion of Human Prostate Cancer Cell by Suppressing Epithelial-Mesenchymal Transition and MMPs Expression
作者:	Shen, Kun-Hung Liao, Alex Chien-Hwa Hung, Jui-Hsiang Lee, Wei-Jiunn Hu, Kai-Chieh Lin, Pin-Tsen Liao, Ruei-Fang Chen, Pin-Shern
貢獻者:	老人服務事業管理系生物科技系
關鍵字:	alpha-solanine invasion EMT matrix metalloproteinase microRNA
日期:	2014-08
上傳時間:	2015-05-06 21:18:28 (UTC+8)
出版者:	Mdpi Ag
摘要:	alpha-Solanine, a naturally occurring steroidal glycoalkaloid found in nightshade (Solanum nigrum Linn.), was found to inhibit proliferation and induce apoptosis of tumor cells. However, the mechanism involved in suppression of cancer cell metastasis by alpha-solanine remains unclear. This study investigates the suppression mechanism of alpha-solanine on motility of the human prostate cancer cell PC-3. Results show that alpha-solanine reduces the viability of PC-3 cells. When treated with non-toxic doses of alpha-solanine, cell invasion is markedly suppressed by alpha-solanine. alpha-Solanine also significantly elevates epithelial marker E-cadherin expression, while it concomitantly decreases mesenchymal marker vimentin expression, suggesting it suppresses epithelial-mesenchymal transition (EMT). alpha-Solanine reduces the mRNA level of matrix metalloproteinase-2 (MMP-2), MMP-9 and extracellular inducer of matrix metalloproteinase (EMMPRIN), but increases the expression of reversion-inducing cysteine-rich protein with kazal motifs (RECK), and tissue inhibitor of metalloproteinase-1 (TIMP-1) and TIMP-2. Immunoblotting assays indicate alpha-solanine is effective in suppressing the phosphorylation of phosphatidylinositide-3 kinase (PI3K), Akt and ERK. Moreover, alpha-solanine downregulates oncogenic microRNA-21 (miR-21) and upregulates tumor suppressor miR-138 expression. Taken together, the results suggest that inhibition of PC-3 cell invasion by alpha-solanine may be, at least in part, through blocking EMT and MMPs expression. alpha-Solanine also reduces ERK and PI3K/Akt signaling pathways and regulates expression of miR-21 and miR-138. These findings suggest an attractive therapeutic potential of alpha-solanine for suppressing invasion of prostate cancer cell.
關聯:	Molecules, v.19 n.8, pp.11896-11914
显示于类别:	[生物科技系(所)] 期刊論文 [高齡福祉養生管理系] 期刊論文

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