Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/28490
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    標題: A novel multiple membrane blood-feeding system for investigating and maintaining Aedes aegypti and Aedes albopictus mosquitoes
    作者: Luo, Yi-Pey
    貢獻者: 生物科技系
    關鍵字: Aedes
    blood feeder
    pig blood
    sausage-casing membrane
    feeding efficiency
    日期: 2014-12
    上傳時間: 2015-05-06 21:18:10 (UTC+8)
    出版者: Soc Vector Ecology
    摘要: A novel multiple membrane blood-feeding system for mosquitoes has been developed for the study and routine maintenance of Aedes aegypti L. and Aedes albopictus Skuse that require a meal of vertebrate blood to produce eggs. This blood-feeding system uses cattle collagen sausage-casing membrane to facilitate feeding. The efficiency of this blood-feeding system was compared to a live mice blood source. We observed that Ae. aegypti that fed on pig whole blood had 89.7% (w/o ATP) and 90.7% (w/ ATP) blood-feeding rates, which were not significantly different from the mice-fed ones (98.0%). Ae. albopictus fed on pig whole blood (w/ ATP) had a success rate of 84.4%, which was significantly different from the mice-fed mosquitoes (51.1%). The feeding rates did not differ between sausage-casing membrane and Parafilm-M (R). The survival rate, fecundity, pupation, and pupal emergence rates of Aedes females fed on pig whole blood were not significantly different from the mice-fed ones. The artificial blood feeder can be applied to replace live animals as blood sources. Considering that this simple, inexpensive, convenient, and efficient feeding device can be built with common laboratory materials for research on Aedes mosquitoes.
    關聯: Journal of Vector Ecology, v.39 n.2, pp.271-277
    显示于类别:[生物科技系(所)] 期刊論文

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