In this project, the major purpose is to investigate of inhibitory effects of bioactive peptides from mackerel on melanin production, hepatocyte oxidative damage and endothelial inflammation. The goal of the first year is to investigate the regulatory effects of bioactive peptides from mackerel on melanin production in B16 cells.However, in this study, the antioxidant and antityrosinase activities of bioactive peptides from mackerel were determined. The antioxidant activities were carried out using different in vitro assays including the inhibition of ABTS and the chelating activity. Bioactive peptides from mackerel displayed antioxidant and antityrosinase activities. In addition, bioactive peptides B2, B4, C2 from mackerel decreased tyrosinase expression in B16 cells. bioactive peptides B2, B4, C2 from mackerel also decrease the levels of TRP-1 and TRP-2 in B16 cells. These data suggested that bioactive peptides B2, B4, C2 from mackerel exhibiting biological activities could contribute to antimelanogenesis as well as antioxidation. 本研究為探討鯖魚生物活性胜肽對黑色素合成、肝臟細胞氧化與內皮細胞發炎之抑制作用，原計畫分成三年完成。第一年計畫為探討鯖魚生物活性胜肽於體外試驗之抗氧化與抗酪胺酸酶效應及其對細胞性黑色素生成之作用。結果顯示，鯖魚生物活性胜肽對ABTS自由基具有抑制作用。此外，鯖魚生物活性胜肽並具有金屬離子螯合之作用。同時，鯖魚生物活性胜肽對酪胺酸酶與B16細胞黑色素合成，皆具有不同程度抑制作用。此外，鯖魚生物活性胜肽B2,B4,C2，對B16細胞產生酪胺酸酶具有抑制作用。鯖魚生物活性胜肽B2,B4,C2並具有酪胺酸酶相關蛋白1與酪胺酸酶相關蛋白2之抑制作用。這些數據顯示，應用鯖魚生物活性胜肽B2,B4,C2可能有助於體內抗黑色素生成和抗氧化作用。
