Reconstitution of the early steps of gliotoxin biosynthesis in Aspergillus nidulans reveals the role of the monooxygenase GliC

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Title:	Reconstitution of the early steps of gliotoxin biosynthesis in Aspergillus nidulans reveals the role of the monooxygenase GliC
Authors:	Chang, Shu-Lin Chiang, Yi-Ming Yeh, Hsu-Hua Wu, Tung-Kung Wang, Clay C. C.
Contributors:	生物科技系藥學系
Keywords:	Biosynthesis Gliotoxin Heterologous Expression
Date:	2013-04
Issue Date:	2014-05-26 10:48:42 (UTC+8)
Publisher:	Pergamon-Elsevier Science Ltd
Abstract:	The gliotoxin, a member of the epipolythiodioxopiperazine (ETP), has received considerable attention from the scientific community for its wide range of biological activity. Despite the identification of gliotoxin cluster, however, the sequence of steps in the gliotoxin biosynthesis has remained elusive. As an alternative to the gene knock-out and biochemical approaches used so far, here we report using a heterologous expression approach to determine the sequence of the early steps of gliotoxin biosynthesis in Aspergillus nidulans. We identified the GliC, a monooxygenases that involved in the second step of gliotoxin biosynthesis pathway through the catalyzing the hydroxylation at the alpha-position of L-Phe. (C) 2013 Elsevier Ltd. All rights reserved.
Relation:	Bioorganic & Medicinal Chemistry Letters, v.23 n.7, pp.2155-2157
Appears in Collections:	[Dept. of Biotechnology (including master's program)] Periodical Articles [Dept. of Pharmacy] Periodical Articles

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