Proteomic Profiling for Peritoneal Dialysate: Differential Protein Expression in Diabetes Mellitus

doi:10.1155/2013/642964

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题名:	Proteomic Profiling for Peritoneal Dialysate: Differential Protein Expression in Diabetes Mellitus
作者:	Yang, Ming-Hui Wang, Hsien-Yi Lu, Chi-Yu Tsai, Wan-Chi Lin, Po-Chiao Su, Shih-Bin Tyan, Yu-Chang
贡献者:	運動管理系
关键词:	Retinol-Binding-Protein Apolipoprotein A-Iv Human Pleural Effusion Alzheimers-Disease Mass-Spectrometry Vitamin-A Type-2 Identification Serum Fluid
日期:	2013
上传时间:	2014-05-26 10:48:36 (UTC+8)
出版者:	Hindawi Publishing Corporation
摘要:	Peritoneal dialysis (PD) is an increasingly accepted modality of renal replacement therapy. It provides the advantages of having a flexible lifestyle, stable hemodynamics, and better preservation of residual renal function. To enhance our understanding of the peritoneal dialysate of diabetesmellitus (DM), peritoneal dialysate proteins were identified by two-dimensional gel electrophoresis (2DE) combined with reverse-phase nano-ultra performance liquid chromatography electrospray ionization tandem mass spectrometry (RP-nano-UPLC-ESI-MS/MS) followed by peptide fragmentation patterning. To validate the differential proteins, ELISA and Western blotting analyses were applied to detect candidate proteins that may be related to DM. We performed 2DE on the peritoneal dialysate samples, with detection of more than 300 spots. From this, 13 spots were excised, in-gel digested, and identified by RP-nano-UPLC-ESI-MS/MS. Ten of these showed significant differential expression between the DM and chronic glomerulonephritis (CGN) peritoneal dialysate samples. In this study, we conducted a comparative proteomic study on these two groups of dialysate that may provide evidence for understanding the different peritoneal protein changes. These proteins may not be new biomarkers; however, they may indicate a situation for possible drug treatment and can be the predictors of peritonitis for a validation study in the future.
關聯:	Biomed Research International,Vol.2013, 642964
显示于类别:	[Dept. of Sports Management] Periodical Articles

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