Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/27895
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    標題: Nuclear Factor-kappa B (NF-kappa B) Regulates the Expression of Human Testis-Enriched Leucine-Rich Repeats and WD Repeat Domain Containing 1 (LRWD1) Gene
    作者: Teng, Yen-Ni
    Chuang, Po-Jung
    Liu, Yo-Wen
    貢獻者: 生物科技系
    關鍵字: Nf-Kappa B
    Lrwd1
    Promoter
    日期: 2013-01
    上傳時間: 2014-05-26 10:48:02 (UTC+8)
    出版者: Mdpi Ag
    摘要: The human Leucine-rich Repeats and WD repeat Domain containing 1 (LRWD1) gene was originally identified by cDNA microarray as one of the genes down-regulated in the testicular tissues of patients with severe spermatogenic defects. Human LRWD1 is a testicular-enriched protein that is present predominantly in the cytoplasm of spermatocytes and spermatids and colocalizes with the centrosome at the base of sperm tail. Reporter assay, Chromatin immunoprecipitation (ChIP) analysis, and gel electrophoretic mobility shift assay (EMSA) were used to identify the core promoter region of LRWD1. A 198 bp segment upstream of the LRWD1 transcription initiation site exhibited promoter activity. The LRWD1 core promoter lacked a TATA box but contained a NF-kappa B binding site. Chromatin immunoprecipitation (ChIP) analysis and gel electrophoretic mobility shift assay (EMSA) showed basal binding of the NF-kappa B subunit to the LRWD1 promoter. LRWD1 promoter activity was positively regulated by NF-kappa B, and this regulation was dependent on the presence of the conserved kappa B site in the LRWD1 promoter region. Our data suggest that NF-kappa B is an important regulator for the expression of LRWD1. This is the first study showing that the expression of the testis-enriched LRWD1 gene is regulated by NF-kappa B.
    關聯: International Journal of Molecular Sciences, v.14 n.1, pp.625-639
    显示于类别:[生物科技系(所)] 期刊論文

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