Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/27852
English  |  正體中文  |  简体中文  |  Items with full text/Total items : 18034/20233 (89%)
Visitors : 23364789      Online Users : 477
RC Version 7.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Tips:
  • please add "double quotation mark" for query phrases to get precise results
  • please goto advance search for comprehansive author search
  • Adv. Search
    HomeLoginUploadHelpAboutAdminister Goto mobile version
    Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/27852


    Title: gamma-Tocotrienol induced cell cycle arrest and apoptosis via activating the Bax-mediated mitochondrial and AMPK signaling pathways in 3T3-L1 adipocytes
    Authors: Wu, Shu-Jing
    Huang, Guang-Yu
    Ng, Lean-Teik
    Contributors: 保健營養系
    Keywords: Tocotrienols
    Apoptosis
    Adipogenesis
    Ampk
    3T3-L1 Adipocytes
    Date: 2013-09
    Issue Date: 2014-05-26 10:46:18 (UTC+8)
    Publisher: Pergamon-Elsevier Science Ltd
    Abstract: This study aimed to examine the anti-proliferative effects of alpha-, gamma- and delta-tocotrienols (alpha T3, gamma T3 and delta T3), and alpha-tocopherol on 3T3-L1 adipocytes. Results showed that compared with other vitamin E analogues, gamma T3 demonstrated the most potent anti-proliferative effect on 3T3-L1 cells. It significantly caused a reduction in mitochondrial membrane potential (Delta psi m) and an increase in ROS formation, as well as inducing cell apoptosis and cell cycle arrest at S phase. Further studies showed that it down-regulated Bcl-2 and PPAR-gamma expression, suppressed Akt and ERK activation and phosphorylation, and caused cytochrome c release from mitochondria to cytosol, whereas it up-regulated CD95 (APO-1/CD95) and Bax expression, and caused caspase-3 and JNK activation, PARP cleavage and AMPK phosphorylation. Pretreatments with caspase-3 (z-DEVD-fmk) and AMPK (CC) inhibitors significantly suppressed the gamma T3-induced ROS production and cell death. Caspase-3 inhibitor also efficiently blocked CD95 (APO-1/CD95) and Bax expression, caspase-3 activation and PARP cleavage, whereas antioxidant N-acetyl-L-cysteine, AMPK inhibitor and AMPK siRNA effectively blocked the AMPK phosphorylation. Taken together, these results conclude that the potent anti-proliferative and anti-adipogenic effects of gamma T3 on 313-L1 adipocytes could be through the Bax-mediated mitochondrial and AMPK signaling pathways. (C) 2013 Elsevier Ltd. All rights reserved.
    Relation: Food And Chemical Toxicology, v.59, pp.501-513
    Appears in Collections:[Dept. of Health and Nutrition (including master's program)] Periodical Articles

    Files in This Item:

    File Description SizeFormat
    index.html0KbHTML1429View/Open


    All items in CNU IR are protected by copyright, with all rights reserved.


    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback