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https://ir.cnu.edu.tw/handle/310902800/27823
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| Title: | Destruxin B Isolated from Entomopathogenic Fungus Metarhizium anisopliae Induces Apoptosis via a Bcl-2 Family-Dependent Mitochondrial Pathway in Human Nonsmall Cell Lung Cancer Cells |
| Authors: | Wu, Chun-Chi
Chen, Tzu-Hsiu
Liu, Bing-Lan
Wu, Li-Chen
Chen, Yung-Ching
Tzeng, Yew-Min
Hsu, Shih-Lan |
| Contributors: | 保健營養系
醫藥化學系 |
| Keywords: | Dna-Damage Response
Antitumor-Activity
Colorectal-Cancer
Natural-Products
Protein Family
Cytochrome-C
Cyclodepsipeptides
Gefitinib
Death
Adenocarcinoma |
| Date: | 2013 |
| Issue Date: | 2014-05-26 10:45:07 (UTC+8) |
| Publisher: | Hindawi Publishing Corporation |
| Abstract: | Destruxin B, isolated from entomopathogenic fungus Metarhizium anisopliae, is one of the cyclodepsipeptides with insecticidal and anticancer activities. In this study, destruxin B was extracted and purified by ion-exchange chromatography, silica gel chromatography, and semipreparative high-performance liquid chromatography. The potential anticancer effects and molecular mechanisms of destruxin B in human nonsmall cell lung cancer cell lines were characterized. Our results showed that destruxin B induced apoptotic cell death in A549 cells. This event was accompanied by the activation of caspase-2, -3, and -9. Moreover, destruxin B increased the expression level of proapoptotic molecule, PUMA, while decreased antiapoptotic molecule Mcl-1. Additionally, the translocation of Bax from cytosol to mitochondrial membrane was observed upon destruxin B treatment. Knockdown of Bax by shRNA effectively attenuated destruxin-B-triggered apoptosis in A549 cells. Interestingly, similar toxic effects and underlying mechanisms including caspase activation, upregulation of PUMA, and downregulation of Mcl-1 were also observed in a p53-null lung cancer H1299 cell line upon destruxin B treatment. Taken together, our findings suggest that destruxin-B-induced apoptosis in human nonsmall cell lung cancer cells is via a Bcl-2 family-dependent mitochondrial pathway. |
| Relation: | Evidence-Based Complementary And Alternative Medicine, v.2013, Article ID 548929 |
| Appears in Collections: | [Dept. of Health and Nutrition (including master's program)] Periodical Articles
[Dept. of Food & Drug Industry and Inspective Technology] Periodical Articles
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