Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/27801
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    標題: Clinicopathological Correlation and Prognostic Significance of Protein Kinase C alpha Overexpression in Human Gastric Carcinoma
    作者: Lin, Shee-Chan
    Chen, Wei-Yu
    Lin, Kai-Yuan
    Chen, Sheng-Hsuan
    Chang, Chun-Chao
    Lin, Sey-En
    Fang, Chia-Lang
    貢獻者: 生物科技系
    關鍵字: Pkc-Alpha
    Hepatocellular-Carcinoma
    Cell-Proliferation
    Messenger-Rna
    Cancer-Cells
    Expression
    Breast
    Invasion
    Marker
    Carcinogenesis
    日期: 2013-02
    上傳時間: 2014-05-26 10:44:13 (UTC+8)
    出版者: Public Library Science
    摘要: Objectives: This study investigated the PKC alpha protein expression in gastric carcinoma, and correlated it with clinicopathological parameters. The prognostic significance of PKC alpha protein expression in gastric carcinoma was analyzed.Methods: Quantitative real-time PCR test was applied to compare the PKC alpha mRNA expression in tumorous and nontumorous tissues of gastric carcinoma in ten randomly selected cases. Then PKC alpha protein expression was evaluated in 215 cases of gastric carcinoma using immunohistochemical method. The immunoreactivity was scored semiquantitatively as: 0 = absent; 1 = weak; 2 = moderate; and 3 = strong. All cases were further classified into two groups, namely PKC alpha overexpression group with score 2 or 3, and non-overexpression group with score 0 or 1. The PKC alpha protein expression was correlated with clinicopathological parameters. Survival analysis was performed to determine the prognostic significance of PKC alpha protein expression in patients with gastric carcinoma.Results: PKC alpha mRNA expression was upregulated in all ten cases of gastric carcinoma via quantitative real-time PCR test. In immunohistochemical study, eighty-eight out of 215 cases (41%) of gastric carcinoma revealed PKC alpha protein overexpression, which was statistically correlated with age (P = 0.0073), histologic type (P < 0.0001), tumor differentiation (P = 0.0110), depth of invasion (P = 0.0003), angiolymphatic invasion (P = 0.0373), pathologic stage (P = 0.0047), and distant metastasis (P = 0.0048). We found no significant difference in overall and disease free survival rates between PKC alpha overexpression and non-overexpression groups (P = 0.0680 and 0.0587). However, PKC alpha protein overexpression emerged as a significant independent prognostic factor in multivariate Cox regression analysis (hazard ratio 0.632, P = 0.0415).Conclusions: PKC alpha protein is upregulated in gastric carcinoma. PKC alpha protein expression is statistically correlated with age, histologic type, tumor differentiation, depth of invasion, angiolymphatic invasion, pathologic stage, and distant metastasis. The PKC alpha protein overexpression in patients with gastric carcinoma is a significant independent prognostic factor in multivariate Cox regression analysis.
    關聯: Plos One, v.8 n.2 pp.e56675
    顯示於類別:[生物科技系(所)] 期刊論文

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