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| 標題: | Characterization of 3-hydroxyisobutyrate dehydrogenase, HIBADH, as a sperm-motility marker |
| 作者: | Tasi, Yung-Chieh
Chao, Hsin-Chih Albert
Chung, Chia-Ling
Liu, Xiu-Ying
Lin, Ying-Ming
Liao, Pao-Chi
Pan, Hsien-An
Chiang, Han-Sun
Kuo, Pao-Lin
Lin, Ying-Hung |
| 貢獻者: | 運動管理系 |
| 關鍵字: | Hibadh
Asthenozoospermia
Sperm Motility
Proteomics |
| 日期: | 2013-04 |
| 上傳時間: | 2014-05-26 10:43:59 (UTC+8) |
| 出版者: | Springer/Plenum Publishers |
| 摘要: | Asthenozoospermia is a major cause of male infertility. However, the molecular mechanisms underlying sperm-motility defects remain largely unknown in the majority of cases. In our previous study, we applied a proteomic approach to identify unknown proteins that were downregulated in spermatozoa with low motility compared to spermatozoa with good motility. Several sperm motility- related proteins have been identified. In this study, 3-hydroxyisobutyrate dehydrogenase (HIBADH), one of the proteins identified using the proteomic tools, is further characterized.Reverse-transcription polymerase chain reactions (RT-PCR), western blotting, and immunofluorescence assays (IFA) were preformed to investigate the expression pattern. The enzymatic activity of HIBADH was evaluated in sperm with good (> 50 %), moderate (< 50 %) and lower motility (< 20 %).Using RT-PCR, we found that transcripts of HIBADH are enriched in the cerebellum, heart, skeletal muscle, uterus, placenta, and testes of male humans. In western blotting, it is expressed in the placenta, testes, and spermatozoa. During spermiogenesis, HIBADH is located at the mid-piece (a specialized development from the mitochondria) of elongating, elongated, and mature sperm. The enzymatic activity of HIBADH in sperm with moderate and lower motility were significantly reduced compared with good motility (P < 0.0001 and P < 0.05, respectively).Our study indicated that HIBADH is involved in the mitochondrial function of spermatozoa, and maintains sperm motility. It may serve as a sperm-motility marker. |
| 關聯: | Journal of Assisted Reproduction And Genetics, v.30 n.4 pp.505-512 |
| 顯示於類別: | [運動管理系] 期刊論文
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