Acid aspiration provokes the pneumonia caused by multidrug-resistant Acinetobacter baumannii in BALB/c mice

doi:10.1016/j.ijid.2013.01.022

CNU IR > Pharmacy and Science > Dept. of Pharmacy > Periodical Articles > Item 310902800/27766

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Title:	Acid aspiration provokes the pneumonia caused by multidrug-resistant Acinetobacter baumannii in BALB/c mice
Authors:	Chiang, Shyh-Ren Tang, Hung-Jen Chen, Chung-Hua Chen, Chi-Chung Lee, Wen-Ying Chang, Ping-Chin Chiu, Yu-Hsin Chuang, Yin-Ching
Contributors:	藥學系
Keywords:	Acid Aspiration Multidrug-Resistant Acinetobacter Baumannii Pneumonia Mice Lung Permeability
Date:	2013-06
Issue Date:	2014-05-26 10:42:48 (UTC+8)
Publisher:	Elsevier Sci Ltd
Abstract:	Objective: To determine whether acid aspiration provokes the development of multidrug-resistant Acinetobacter baumannii (MDRAB) pneumonia in its host.Methods: Groups of mice were inoculated intratracheally (IT) with 50 mu l of 0.1 N HCl and 1 x 10(8) colony-forming units (CFU) Ab396 (A + Ab group), or 50 mu l of 0.1 N HCl and 20 mu l of 0.9% saline (A + S group), or 20 mu l of 0.9% saline and 1 x 10(8) CFU of Ab396 (S + Ab group), or 50 mu l of 0.9% saline and 20 mu l of 0.9% saline (S + S group). Cytokines, bacterial loads in the bronchoalveolar lavage fluid (BALF), lung permeability, histopathology of the lungs, and survival rates were evaluated.Results: Only the A + Ab mice developed extensive Ab396 pneumonia and had significantly elevated bacterial loads, increased lung leakage, and lower levels of tumor necrosis factor alpha (TNF-alpha) compared with the other three groups (p < 0.05, Mann-Whitney U-test). Moreover, a strong synergistic effect (p < 0.05, two-way analysis of variance) was observed between the acid induction and Ab396 infection, resulting in lung injury and an unfavorable survival outcome.Conclusions: Lung injury caused by acid aspiration provoked secondary MDRAB pneumonia; also synergistic effects between acid aspiration and Ab396 infection resulted in a detrimental outcome in the infected mice. (C) 2013 International Society for Infectious Diseases. Published by Elsevier Ltd. All rights reserved.
Relation:	International Journal of Infectious Diseases, v.17 n.6 pp.E454-E460
Appears in Collections:	[Dept. of Pharmacy] Periodical Articles

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