New Cationic Biodegradable Poly(Urethane-co-Ester): Synthesis, Structural Characterization, Modification and Gene Delivery

doi:10.1163/092050610X541935

Chia Nan University of Pharmacy & Science Institutional Repository > 藥理學院 > 生物科技系(所) > 期刊論文 > Item 310902800/27730

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://ir.cnu.edu.tw/handle/310902800/27730

標題:	New Cationic Biodegradable Poly(Urethane-co-Ester): Synthesis, Structural Characterization, Modification and Gene Delivery
作者:	Chang, Wei-Yang Hsiao, Yu-Che Shau, Min-Da
貢獻者:	生物科技系
關鍵字:	Poly(Urethane-Co-Ester) Binary Mixture Blending Method Buffering Capacity Transfection Efficiency
日期:	2012
上傳時間:	2014-03-24 15:23:03 (UTC+8)
出版者:	Vsp Bv
摘要:	To improve the transfection efficiency of poly(urethane-co-ester) and the cytotoxicity of PEI25k with DNA, we synthesized a new poly(urethane-co-ester), PUE, bearing ester linkages and amino groups in the backbone and urethane linkages in the side-chain, and then prepared a binary mixture, PUE-PEI25k, using a physical blending method. The structure of PUE was confirmed by FT-IR and NMR spectra. Both poly(urethane-co-ester), PUE, and binary mixture PUE-PEI25k, readily self-assembled with plasmid DNA (pCMV-beta gal) in a HEPES buffer, were characterized by dynamic light scattering. The results revealed that PUE and PUE-PEI25k were able to self-assemble plasmid DNA into PUE/DNA and PUE-PEI25k/DNA nano-complexes small enough to enter a cell through endocytosis. Titration studies were performed to determine the buffering capacities of PUE and PUE-PEI25k. The COS-7 cell viability in the presence of PEI25k, PUE and PUE-PEI25k was studied. At low mass ratio of PUE/PEI25k (150:1), it was found that the PUE-PEI25k/DNA complexes were able to transfect COS-7 cells in vitro with a high efficiency comparable to a well-known gene carrier, PEI25k/DNA. The results indicate that the binary mixture PUE-PEI25k is an attractive cationic carrier for gene delivery and an interesting candidate for further study. (C) Koninklijke Brill NV, Leiden, 2012
關聯:	Journal of Biomaterials Science-Polymer Edition, 23(1-4), 27-41
显示于类别:	[生物科技系(所)] 期刊論文

文件中的档案:

档案	描述	大小	格式	浏览次数
index.html		0Kb	HTML	1533	检视/开启

在CNU IR中所有的数据项都受到原著作权保护.

TAIR相关文章

数据加载中.....