Bioactive constituents and anti-proliferative properties of supercritical carbon dioxide Salvia miltiorrhiza extract in 3T3-L1 adipocytes

doi:10.1016/j.procbio.2011.10.034

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標題:	Bioactive constituents and anti-proliferative properties of supercritical carbon dioxide Salvia miltiorrhiza extract in 3T3-L1 adipocytes
作者:	Wu, Shu-Jing Lee, Shu-Jung Su, Chun-Han Lin, Doung-Liang Wang, Shyh-Shyan Ng, Lean-Teik
貢獻者:	保健營養系
關鍵字:	Salvia Miltiorrhiza Supercritical Carbon Dioxide Ros Ppar-Gamma Mapk Ampk
日期:	2012-02
上傳時間:	2014-03-21 16:17:46 (UTC+8)
出版者:	Elsevier Sci Ltd
摘要:	The present study examined the anti-proliferative properties of aqueous (WESM), ethanolic (EESM) and supercritical carbon dioxide (SCESM-5) extracts of Salvia miltiorrhiza in 3T3-L1 adipocytes, and the contents of bioactive compounds salvianolic acid B (SAB), cryptotanshinone (CTS), tanshinone 1 (TS-1) and tanshinone IIA (TS-IIA). Results showed that WESM contained mainly SAB. whereas EESM possessed all the four bioactive compounds, but its concentrations of CTS, TS-1 and TS-IIA were 2-3-fold lower than SCESM-5. SCESM-5 demonstrated the most potent inhibitory effect on 3T3-L1 cell proliferation, and induction of cell apoptosis and cell cycle arrest. Further studies showed that it enhanced intracellular ROS level, down-regulated CD95 (APO-1/CD95), Bcl-2 and PPAR-gamma, up-regulated Bax, AMPK and p-AMPK, and caused activation of caspase-3 and PARP cleavage, as well as JNK and ERK activation and phosphorylation. Pretreatments with antioxidant (Vit E), caspase-3 (z-DEVD-fmk) and ERK (PD98059) inhibitors significantly suppressed the SCESM-5-induced ROS production and cell death. Caspase-3 inhibitor efficiently blocked the Bax expression, caspase-3 activation and PARP cleavage, while ERK inhibitor suppressed the ERK phosphorylation after SCESM-5 treatment. This study concludes that CTS. TS-1 and TS-IIA of SCESM-5 may have contributed to the potent anti-proliferative activity through the MAPK and AMPK pathways in 3T3-L1 adipocytes. (C) 2011 Elsevier Ltd. All rights reserved.
關聯:	Process Biochemistry, 47(2), 216-224
顯示於類別:	[保健營養系(所) ] 期刊論文

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