Screening of New Microsatellite DNA Markers from the Genome of Platyeriocheir formosa

doi:10.3390/ijms13055598

Chia Nan University of Pharmacy & Science Institutional Repository > 環境永續暨休閒學院 > 環境資源管理系(所) > 期刊論文 > Item 310902800/27653

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標題:	Screening of New Microsatellite DNA Markers from the Genome of Platyeriocheir formosa
作者:	Cheng, Hui-Ling Lee, Yan-Horn Hsiung, Dai-Shion Tseng, Mei-Chen
貢獻者:	環境資源管理系
關鍵字:	Catadromous Crab Cross-Specific Amplification Polymorphic Loci
日期:	2012-05
上傳時間:	2014-03-21 16:16:34 (UTC+8)
出版者:	Mdpi Ag
摘要:	The catadromous Platyeriocheir formosa is a crab endemic in Taiwan. To conserve P. formosa population diversity and ensure the sustainable use of this natural resource, we have developed new genetic markers, 17 polymorphic microsatellite loci, to promote the study of its population genetics in the future. In this study, more than 70 microsatellite sequences were found. Among these, 18 loci were selected to analyze the genetic diversity of P. formosa. With the exception of the Pfo15 locus, all of the remaining loci were polymorphic with allelic numbers ranging from 3-14. Heterozygosity within all 17 polymorphic loci ranged from 0.2-0.95 with an average of 0.55, which suggested that these loci are proper markers for studying population genetics. After we tested cross-specific amplification, eight and six primer sets could be successfully used for the amplification of microsatellite loci in morphologically similar Eriocheir sinensis and E. japonica, respectively; this suggests that they are useful markers for closely related species.
關聯:	International Journal of Molecular Sciences, 13(5), 5598-5606
显示于类别:	[環境資源管理系(所)] 期刊論文

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