A one-step process in preparation of cationic nanoparticles with poly(lactide-co-glycolide)-containing polyethylenimine gives efficient gene delivery

doi:10.1016/j.ejps.2012.04.006

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標題:	A one-step process in preparation of cationic nanoparticles with poly(lactide-co-glycolide)-containing polyethylenimine gives efficient gene delivery
作者:	Shau, Min Da Shih, Mei Fen Lin, Chi Cheng Chuang, I.Chuan Hung, Wei Chih Hennink, Wim E. Cherng, Jong Yuh
貢獻者:	生物科技系藥學系
關鍵字:	Cytotoxicity Transfection Plga Pei`
日期:	2012-08-15
上傳時間:	2014-03-21 16:14:27 (UTC+8)
出版者:	Elsevier Science Bv
摘要:	A one-step preparation of nanoparticles with poly(lactide-co-glycolide) (PLGA) pre-modified with polyethylenimine (PEI) is better in requirements for DNA delivery compared to those prepared in a two-step process (preformed PLGA nanoparticles and subsequently coated with PEI). The particles were prepared by emulsification of PLGA/ethyl acetate in an aqueous solution of PVA and PEI. DLS, AFM and SEM were used for the size characteristics. The cytotoxicity of PLGA/PEI nanoparticles was detected by mu assay. The transfection activity of the particles was measured using pEGFP and p beta-gal plasmid DNA. Results showed that the PLGA/PEI nanoparticles were spherical and non-porous with a size of about 0.2 mu m and a small size distribution. These particles had a positive zeta potential demonstrating that PEI was attached. Interestingly, the zeta potential of the particles (from one-step procedure) was substantially higher than that of two-step process and is ascribed to the conjugation of PEI to PLGA via aminolysis. The PLGA/PEI nanoparticles were able to bind DNA and the formed complexes had a substantially lower cytotoxicity and a higher transfection activity than PEI polyplexes. In conclusion, given their small size, stability, low cytotoxicity and good transfection activity, PLGA/PEI-DNA complexes are attractive gene delivery systems. (c) 2012 Elsevier B.V. All rights reserved.
關聯:	European Journal of Pharmaceutical Sciences, 46(5), 522-529
顯示於類別:	[生物科技系(所)] 期刊論文 [藥學系(所)] 期刊論文

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