Antiproliferative Activity and Apoptosis Induction of Eucalyptus Citriodora Resin and Its Major Bioactive Compound in Melanoma B16F10 Cells

doi:10.1021/jf301068z

CNU IR > Pharmacy and Science > Dept. of Food Science & Technology > Periodical Articles > Item 310902800/27587

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/27587

Title:	Antiproliferative Activity and Apoptosis Induction of Eucalyptus Citriodora Resin and Its Major Bioactive Compound in Melanoma B16F10 Cells
Authors:	Duh, Pin-Der Chen, Zong-Tsi Lee, Shwu-Woan Lin, Tsuey-Pin Wang, Ya-Ting Yen, Wen-Jye Kuo, Ling-Feng Chu, Heuy-Ling
Contributors:	食品科技系醫藥化學系保健營養系
Keywords:	B16F10 Melanoma Cell Eucalyptus Citriodora Resin Oxidative Stress Apoptosis 6-[1-(P-Hydroxy-Phenyl)Ethyl]-7-O-Methyl Aromadendrin Antiproliferation
Date:	2012-08-15
Issue Date:	2014-03-21 16:14:25 (UTC+8)
Publisher:	Amer Chemical Soc
Abstract:	Antiproliferative activity and apoptosis induction of ethyl acetate of Eucalyptus citriodora resin (EAEER), and its major bioactive compound in melanoma B16F10 cells were investigated. 6-[1-(p-Hydroxy-phenyl)ethyl]-7-O-methyl aromadendrin (HEMA), a flavanol derivative, was isolated from EAEER and identified on the basis of its mass and NMR spectra. The results from MTT assay showed high antiproliferative effects of EAEER and HEMA on B16F10 cells. Moreover, EAEER- and HEMA-induced cell apoptosis was association with the decrease in the mitochondrial transmembrane potentials (Delta psi(m)), increase in Bax/Bcl-2 ratio, and activation of caspase-3. Cells treated with EAEER and HEMA generated intracellular reactive oxygen species (ROS) and nitric oxide (NO), indicating that ROS and RNS play important roles in the induction of apoptosis in B16F10 cells. Taken together, EAEER and its major bioactive compound, HEMA, inhibited the proliferation of B16F10 cells via apoptosis and may be a potential antimelanoma agent.
Relation:	Journal of Agricultural And Food Chemistry, 60(32), 7866-7872
Appears in Collections:	[Dept. of Food Science & Technology] Periodical Articles [Dept. of Food & Drug Industry and Inspective Technology] Periodical Articles [Dept. of Health and Nutrition (including master's program)] Periodical Articles

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