Chia Nan University of Pharmacy & Science Institutional Repository:Item 310902800/27556
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    Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/27556


    Title: Overexpression of the Aspergillus nidulans histone 4 acetyltransferase EsaA increases activation of secondary metabolite production
    Authors: Soukup, Alexandra A.
    Chiang, Yi-Ming
    Bok, Jin Woo
    Reyes-Dominguez, Yazmid
    Oakley, Berl R.
    Wang, Clay C.C.
    Strauss, Joseph
    Keller, Nancy P.
    Contributors: 藥學系
    Keywords: Ribosomal-Protein Genes
    Biosynthetic-Pathway
    Filamentous Fungi
    Sclerotial Production
    Promoter Nucleosomes
    Sexual Development
    Acetylation
    Yeast
    Cluster
    Recruitment
    Date: 2012-10
    Issue Date: 2014-03-21 16:13:23 (UTC+8)
    Publisher: Wiley-Blackwell
    Abstract: Regulation of secondary metabolite (SM) gene clusters in Aspergillus nidulans has been shown to occur through cluster-specific transcription factors or through global regulators of chromatin structure such as histone methyltransferases, histone deacetylases, or the putative methyltransferase LaeA. A multicopy suppressor screen for genes capable of returning SM production to the SM deficient ?laeA mutant resulted in identification of the essential histone acetyltransferase EsaA, able to complement an esa1 deletion in Saccharomyces cereviseae. Here we report that EsaA plays a novel role in SM cluster activation through histone 4 lysine 12 (H4K12) acetylation in four examined SM gene clusters (sterigmatocystin, penicillin, terrequinone and orsellinic acid), in contrast to no increase in H4K12 acetylation of the housekeeping tubA promoter. This augmented SM cluster acetylation requires LaeA for full effect and correlates with both increased transcript levels and metabolite production relative to wild type. H4K12 levels may thus represent a unique indicator of relative production potential, notably of SMs.
    Relation: Molecular Microbiology, 86(2), 314-330
    Appears in Collections:[Dept. of Pharmacy] Periodical Articles

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