Optimisation of an Ultrasound-assisted Extraction Followed by RP-HPLC Separation for the Simultaneous Determination of Oleanolic Acid, Ursolic Acid and Oridonin Content in Rabdosia rubescens

doi:10.1002/pca.2365

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標題:	Optimisation of an Ultrasound-assisted Extraction Followed by RP-HPLC Separation for the Simultaneous Determination of Oleanolic Acid, Ursolic Acid and Oridonin Content in Rabdosia rubescens
作者:	Yang, Yu-Chiao Wei, Ming-Chi Huang, Ting-Chia
貢獻者:	環境工程與科學系
關鍵字:	Hplc Oridonin Oleanolic Acid Ursolic Acid Isodon Rubescens Rabdosia Rubescens
日期:	2012-11
上傳時間:	2014-03-21 16:12:52 (UTC+8)
出版者:	Wiley-Blackwell
摘要:	Introduction Rabdosia rubescens is a commonly used herb in traditional Chinese medicine and contains diterpenoids, triterpenoids and various other compounds. Among these components, oridonin, oleanolic acid and ursolic acid have gained considerable interest concerning anti-cancer activities. However, there is no suitable currently available method for the simultaneous evaluation of these three bioactive compounds in R. rubescens. Objective To develop an optimised ultrasound-assisted extraction (UAE) method and an efficient HPLC method for the simultaneous evaluation of the three bioactive compounds in R. rubescens. Methodology Various parameters that can potentially affect the UAE process were investigated and optimised. HPLC operating conditions were also optimised, and the chromatographic separation was performed on a C-18-column with an acetonitrilewater gradient as the mobile phase. Results Validation of the HPLC technique developed showed that the method has good linearity, sensitivity, precision and accuracy. The combined UAEHPLC method was applied to quantitate the amount of oridonin, oleanolic acid and ursolic acid in the plant sample and exhibited good repeatability (RSD (%)<3.9), reproducibility (RSD (%)<5.9), stability (RE (%)<1.4) and recovery (mean (%)>92.4). Furthermore, the combined UAEHPLC method was applied successfully to the extraction and determination of oridonin, oleanolic acid and ursolic acid content in samples obtained from different geographical regions, which is the first time that this comparison has been investigated. Conclusion The combined UAEHPLC process is a fast, convenient and appropriate method for the quantitative analysis of these three compounds in R. rubescens. Copyright (c) 2012 John Wiley & Sons, Ltd.
關聯:	Phytochemical Analysis, 23(6), 627-636
显示于类别:	[環境工程與科學系(所)] 期刊論文

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