由於電腦的廣泛使用，舉凡資料處理、電子郵件、電子書籍等，都需要經使用電腦。長時間的接觸電腦終端機（Visual Display Terminal; VDT），使用者的視覺負荷也隨著提高，視覺疲勞的問題日趨嚴重，有關視覺疲勞之量測成為普遍關注的議題。本研究之目的在於使用腦波（Electroencephalogram；EEG）及閃光融合閾值（Critical Fusion Frequency ; CFF）來評估視覺疲勞。以電腦模擬賽車遊戲做為VDT視覺疲勞實驗，研究對象為大學日間部學生，實驗前先填寫受測同意書及NASA工作負荷評估量表（NASA Task Load Index; NASA-TLX），並進行五分鐘EEG及CFF測做為基準值間，隨後進行15分鐘賽車遊戲並全程量測其EEG，賽車遊戲後緊接著測量其CFF，完成一個階段之實驗，共需完成四個階段，整個實驗約1小時30分鐘，實驗最後再填寫一次NASA工作負荷評估量表。測試結果發現經過第一階段賽車遊戲之後，受測者之CFF值可較早偵測到疲勞現象，而受測者之EEG右視覺區（O2）之/（覺醒水準）第一階段及第三階段與基準值間都有顯著上升，感覺區（Pz）之/（覺醒水準）在第一階段與基準值間有顯著上升隨後又下降。而左視覺區（O1）位置之（+）/（睏倦水準）第一階段與基準值間顯著下降，顯示賽車遊戲初期由於需專注於遊戲所以EEG量測結果呈現較高之覺醒水準，而評估疲勞之波在枕葉區（O1及O2）從第一階段到第二階段有顯著上升亦即視覺區較早產生疲勞現象，慢慢延伸至感覺區（Pz）。由NASA工作負荷評估量表也顯著增加，顯現受測者經歷1小時之賽車遊戲已自覺疲勞感。綜合上述實驗量測結果，本研究發現閃光融合閾值對初期之視覺疲勞有較佳之偵測能力，而腦波則可量測到較後期之疲勞現象。 The uses of computers in modern society are omnipresent, including document process, E-mail, electronic book. The visual fatigue problems are going serious gradually, as computer users contact with visual display terminals （VDT） in long time. The measurements of visual fatigue become a concerned issue. This study aims to evaluate visual fatigue using electroencephalogram （EEG） and critical fusion frequency （CFF）. The computer simulation race car game was used to serve as VDT task. The college students were recruited to participate in this study. Before test, the participants filled out the protocols and NASA Task Load Index （NASA-TLX） rating scales and then the baseline of their CFF and EEG were measured for 5 min. The participants played the race car game for 15 min and their EEG was measured at the same time. After 15 min of race car game, their CFF was measured immediately and the stage 1 of the experimental test finished. There were 4 stages of the experimental test in this study. It spends about one and a half hours to undertake the all experimental test. After 4 stages of test the NASA-TLX rating scales were filled out again by the participants. The findings are the value of CFF could detect the visual fatigue after stage 1 of race car game; the alert level （/） of EEG measurement at O2 recording sites increased at stage 1 and stage 3 in comparison with baseline, the alert level（/）of EEG measurement at Pz recording sites increased at stage 1 then decreased; the drowse level（+）/）of EEG measurement at O2 recording sites decreased at stage 1 in comparison with baseline. It reveals the participants need to pay attention to play race car game with higher alert level. The wave of EEG used for fatigue assessment was found increased at stage 1 and stage 2 in comparison with baseline, i.e. fatigue phenomenon induced from visual tasks in visual area and extend to sensory area. For NASA-TLX rating scales results shows the participants essentially felt fatigue undertook 1-hour race car game. Summary the results, we find CFF measurement is sensitive to detect the early visual fatigue, while EEG measurement suitable to assess the later fatigue phenomenon.