牛樟芝 (Antrodia cinnamomea)為台灣特有種真菌，以保健功效聞名，其中包含提升人體免疫力、降低肝脂質過氧化、抗癌及抗發炎等功效。漆氧化酶 (EC 1.10.3.2) 由日本人首於漆樹中發現，也廣泛存在於真菌、高等植物、昆蟲與細菌中，具有淨化工業污水、紙漿漂白等功能。本實驗室先前研究得知牛樟芝亦具分泌漆氧化酶之能力。本研究發現培養一至十四天之牛樟芝發酵液中漆氧化酶活性隨著培養時間增長而逐漸增強；其液態發酵液之pH值於培養第一天至第九天快速下降，第九天後則又逐漸上升，但仍維持於弱酸性；液態培養菌絲體型態由乳麋狀隨著培養時間增長呈顆粒狀，菌絲體乾重也逐日增加。此研究顯示隸屬褐腐菌的台灣特有真菌牛樟芝，可分泌漆氧化酶，其所具有的生物活性有待釐清，希望未來可提供欲大量培養牛樟芝以供應用之依據。 The enzyme activity of laccase was detected in a variety of molds, insects, some plants, and bacteria. It also has been purified from cultured mycelia of various mushrooms. Studies of laccase have been carried out owing to biocatalysts for industrial effluents, and paper pulp bleaching. In our previous study, the results indicated that the activity of laccase in Antrodia cinnamomea, a special kind of fungi in Taiwan, was detected under environmental treatments. In this study, the results showed that the laccase activity was increased from 7 to 14 days in liquid culture, and the pH value of liquid medium of A. cinnamomea was decreased from 1 to 9 days, than increased from 10 to 14 days. The mycelial morphology was chylous at initial stage, than became granulose gradually. These results could provide a great reference for mass production of A. cinnamomea.
