脂肪酸1,1,3,3-四甲基胍鹽的合成與抗菌性能之研究

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Title:	脂肪酸1,1,3,3-四甲基胍鹽的合成與抗菌性能之研究 Synthesis and antimicrobial activity of 1,1,3,3-tetramethylguanidinium aliphatate
Authors:	詹凱淇
Contributors:	化粧品應用與管理系暨化粧品科技研究所何文岳
Keywords:	脂肪酸胍紙錠擴散試驗最低抑菌濃度最低殺菌濃度 1,1,3,3-tetramethylguanidium aliphatate disc-diffusion test Minimum inhibitory concentration Minimum microbicidal concentration
Date:	2013
Issue Date:	2014-03-07 13:55:38 (UTC+8)
Abstract:	脂肪酸是由烴類基團連結羧基所構成的羧酸化合物，也是化妝品中重要的原料之一，部分脂肪酸會對細菌或真菌等微生物產生抗菌效能，但因溶解度不佳的問題，導致使用上的不方便。此研究利用1,1,3,3-四甲基胍(1,1,3,3-tetramethylguanidine)結合不同碳鏈的脂肪酸反應後，得到九種不同脂肪酸胍離子液體，探討其溶解度，並經由(a)紙錠擴散試驗法;(b)最小抑菌濃度(Minimum inhibitory concentration, MIC);(c)最小殺菌濃度(Minimum microbicidal concentration, MMC)等方法來評估抗菌的能力。由實驗結果顯示，脂肪酸胍無論碳鏈長短，對水都有極佳的溶解度，至於抗菌活性，由紙錠擴散結果顯示，C8~C12的脂肪酸胍有較佳的抗菌效果，其中1,1,3,3-tetramethylguanidium dodecanoate在濃度20 mg/mL時對金黃色葡萄球菌及枯草桿菌的抑菌圈分別達到23 mm及22.3 mm，其MIC值分別為142.6 µg/mL及189.0 µg/mL，MMC值分別為227.3 µg/mL及254.0 µg/mL。對於大腸桿菌、綠膿桿菌的抑菌圈約為12 mm，其 MIC與 MMC值效果皆大於1000 µg/mL，而白色念珠菌的抑菌圈可達到11 mm，其MIC值約為325.3 µg/mL，MMC值約489.6 µg/mL。 Fatty acid is a carboxylic acid with a long saturated or unsaturated aliphatic chain. It is a main ingredient used in cosmetic products. Part of fatty acids show the antimicrobial efficacy of microorganisms such as bacteria or fungi, but the problem of solubility leads to use of inconvenience.This study utilized 1,1,3,3-tetramethylguanidine to react with different carbon chain length of fatty acid, producing nine 1,1,3,3-tetramethylguanidium aliphatate (TGA). Antimicrobial activity of the TGA was evaluated with (a) disc diffusion assay; (b)minimum inhibitory concentration, (MIC); (c)minimum microbicidal concentration, (MMC) to evaluate the antibacterial capacity.This study showed that the different types of TGA should be dissolved in water. According to our experimental results revealed that the antibacterial activity had not obvious effect in C8~C12 carbon chains of TGA. For example, 1,1,3,3-tetramethylguanidium dodecanoate at 20 mg/mL could inhibit the Staphylococcu aureus and Baccillus Subtilis growth to 23 mm and 22.3 mm of inhibition zones (MIC, 142.6 �g/mL and 189.0 �g/mL ; MMC, 227.3 �g/mL and 254.0 �g/mL, respectively), the inhibition zones of Escherichia coli and Pseudomonas aeruginosa were about 12 mm (MIC >1000 �g/mL, MMC >1000 �g/mL, respectively), the growth inhibition zones was about 11 mm (MIC 325.3 �g/mL and MMC 489.6 �g/mL, respectively) in Candida albicans.
Relation:	電子全文公開日期：20160724，學年度：101，91頁
Appears in Collections:	[Dept. of Cosmetic Science and institute of cosmetic science] Dissertations and Theses

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