隨著化粧品產業的蓬勃發展與科技發達，消費者對於化粧品的期許已非僅止於功效性，其安全性及相關資訊也更被重視。化粧品配方富含許多營養物質，添加抗菌劑有其必要性，但目前抗菌劑大多著重於抑菌功效之評估，對於抑菌機制則相對未被深入探討，另外有鑒於微生物抗藥性亦趨嚴重，本研究將針對革蘭氏陰性菌細胞外膜蛋白C做為探討，因其影響抑菌機制與抗藥性均佔有重要功能，故期望提供一個檢驗平台能更加以瞭解抑菌機制與監控其抗藥性變化。本實驗探討化粧品常用抗菌劑，對-羥基苯甲酸甲酯、苯氧乙醇、咪唑丁尿素，與兩種雙咪唑陽離子結構離子液體IL-1 、IL-2於0.2 %、0.4 %、0.8 %與大腸桿菌反應6小時後對其外膜蛋白C表現量的影響，實驗過程中觀察到部分隨抗菌劑添加濃度增加所蒐集之菌量與RNA之抽取量有下降趨勢，但藉由反轉錄聚合酶鏈鎖反應方法檢視其外膜蛋白C訊息RNA表現量，結果顯示實驗中所用的三種抗菌劑，對-羥基苯甲酸甲酯、苯氧乙醇、咪唑丁尿素，與雙咪唑陽離子結構離子液體IL-1 、IL-2，均對於外膜蛋白C訊息RNA表現量並未有明顯改變。 For the rapid development of cosmetic industry, customers hope higher safety for the cosmetics and they look forward to understanding more relative information than before.Addition of the preservatives is necessary for preventing the bacterial pollution on cosmetic ingredients. However, the antibacterial mechanism does not understand yet. This study focuses these preservatives on antibacterial mechanism instead of the antibacterial efficacy and expects to provide an inspection platform to know the mechanism of bacteriostasis. In this study, the Escherichia coli was used to react with 0.2 %, 0.4 % and 0.8 % preservatives for 6 hours and detected the effect of the gene expression of outer membrane protein C. Our results exhibited that the amount of bacterial mRNA decreased when the concentration of the preservatives increased. But there were no obvious changes on the mRNA amount of outer membrane protein C by the technique of reverse transcriptase polymerase chain reaction when the different preservatives and antibacterial agents-methyl paraben, phenoxyethanol, imidazolidinyl urea, IL-1, and IL-2 were added in E. coli.
