本研究針對化粧品中荷爾蒙物質利用高效能液相層析法(HPLC)建立兩種不同分析方法，第一部分為分析Estradiol (E2) 和 Ethinyl estradiol (EE2)，以高效能液相層析儀連接螢光偵測器以提高其感度，由研究結果顯示雌激素成分之標準曲線之線性範圍為0.05-1.0 μg/mL，相關係數(R2)皆達於0.9999，評估同日內及異日間之相對標準偏差均低於0.7%。本研究於自製樣品之添加回收率介於97.31-99.75%，相對標準偏差均低於2.34%。第二部分使用微胞液相層析(MLC)分析化粧品中未允許添加之四種荷爾蒙成分，由研究結果顯示其類固醇成分之標準曲線之線性範圍為5-100 μg/mL，相關係數(R2)皆達於0.9995，評估同日內及異日間之相對標準偏差均低於0.8%；於自製樣品之添加回收率介於96.95-99.75%，相對標準偏差均低於4.19%。 This study is to establish two difference analytic methods for hormones in cosmetics by high performance liquid chromatographic (HPLC). The first one is to analyse estradiol (E2) and Ethinyl estradiol (EE2) by HPLC with fluorescence detector was used to increase the sentivity. The result showed good linearity is also achieved in the range of 0.05-1.0 μg/mL with a linear coefficient R2 of 0.9999. Excellent method reproducibility was found for intra- and inter-day precisions with relative standard deviations of <0.7%. The spike of samples are ranging from 97.31 to 99.75%, with standard deviations (RSD) less than 2.34%. The second attempt is to analyse four illegal hormones in cosmetics by Micellar liquid chromatography (MLC) method. The results showed good linearity was achieved in the range of 5-100 μg/mL with linear coefficient R2 higher than 0.9995. The spike of samples are ranging from 96.95 to 99.75%, with standard deviations (RSD) less than 4.19%.
