Preparation of H.C.G.. -Latex and Study of Gonadotropin-like protein n early pregnant placenta and Preparation of. Reagent for Diagnosing Pregnancy in women.

CNU IR > Chna Nan Annual Bulletin > Vol.6 (1980) > Item 310902800/27123

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Title:	Preparation of H.C.G.. -Latex and Study of Gonadotropin-like protein n early pregnant placenta and Preparation of. Reagent for Diagnosing Pregnancy in women. H.C.G-latex之製造及胎盤絨毛性腺激素之研究與檢定婦女懷孕試藥之試劑
Authors:	吳韻秋李秀瀧梁逸
Contributors:	私立嘉南藥學專科學校台灣省畜產試驗所加工系
Date:	1980
Issue Date:	2013-12-19 11:33:59 (UTC+8)
Abstract:	An attempt was made to use a HCG-like protein extracted from an early prognant placenta for the preparation of a raegent for diagnosing pregnancy in women. Although the exact function of the HCG-like protein in early pregnancy Is not definitely known, we found that there was at least seven components which showed antigenic behavior in inhibiting the agglutinating reaction between HCG-latex and HCG-antiserum. The fraction which contained the major HCG activity in placenta has been isolated, partially characterized, and applied to produce antiserum and HCG-latex. The carboxylated latex with a diameter of 0.31u, made from polymerization of styrene and acrylic acid, was charaterized and used to prepare HCG-latex. The use of water-soluble carbodiimide reagents provided an easy and rapid method of coupling HCG to carboxylated latex in 0.15M phosphate buffer, pH 7.3, at room temperature. The basis of the reaction for a positive pregnancy diagnosis is absence of agglutination of HCG-latex and HCG.-antiserum when either urinary HCG or. placenta HCG is added. 由早期胎盤中提煉出類似絨毛腺性激素荷爾蒙(Human Chorionic Gonadotropin，以下簡寫HCG.)應用在調製檢定婦女懷孕試葯。胎盤絨毛組織每公斤提煉45萬單位相當於絨毛激素荷爾蒙。其中有7種類似腺激素荷爾蒙(Gonadotropin)作用的成分。抽取並純化活性最高，含量最多的一塊，(其分子量27,500)。腺激素荷爾蒙活性胎盤中的70％且性狀與尿中抽取之HCG相似，在pH3以下，活性盡失，但分子量不同，氨基酸之構造也有很多出入。對濃尿素的耐力也相似在8M。濃度尿素中仍有50％的活力。這種純化成份，注射免體製造的抗血清(HCG-Antiserum)與由尿中抽出純化HCG的抗血清，相同的對HCG-latex有凝集反應。此抗體平均可保存一年以上，一年半後迅速失去活力，不再有凝集作用。另由Styrene與acrylic acid在水中乳化聚合製成含有碳酸基高分子乳狀樹脂(Carboxylic latex)其大小約為0.31μ，而每21個分子Styrene中有一個碳酸基，經試驗後效果很好。樹脂乳體中之碳酸基與荷爾蒙中之氨基在水溶性Carbodimide存在下反應，結合腺激素荷爾蒙樹脂乳液經過數次高速分離機純化的腺激素HCG-latex。HCG-latex和HCG Antiserum混合即有凝集、沉澱作用。但懷孕婦女尿中之HCG與上列兩物混合時可阻止其凝集作用，以此試葯檢驗，婦女之懷孕與否可達100％效果。
Relation:	嘉南學報 6 : (轉載論文) A39-A58 應用介面化學第3卷第3期 (1980)
Appears in Collections:	[Chna Nan Annual Bulletin] Vol.6 (1980) [Dept. of Pharmacy] Periodical Articles

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