Protective effects of Chlorella-derived peptide on UVB-induced production of MMP-1 and degration of procollagen genes in human skin fibroblasts

doi:10.1016/j.yrtph.2011.03.001

CNU IR > Pharmacy and Science > Dept. of Pharmacy > Periodical Articles > Item 310902800/27073

Please use this identifier to cite or link to this item: https://ir.cnu.edu.tw/handle/310902800/27073

Title:	Protective effects of Chlorella-derived peptide on UVB-induced production of MMP-1 and degration of procollagen genes in human skin fibroblasts
Authors:	Chen, Chiu-Lan Liou, Shu-Fen Chen, Su-Jong Shih, Mei-Fen
Contributors:	藥學系
Keywords:	UVB MMP-1 CYR61 Procollagen TbRII;c-fos c-jun MCP-1 Skin fibroblasts Chlorella
Date:	2011-06
Issue Date:	2013-10-25 15:33:50 (UTC+8)
Publisher:	Elsevier
Abstract:	UV exposure is known to induce premature aging, which is mediated by matrix metalloproteinase-1 (MMP-1) activity. MMP-1 mRNA expression is up-regulated by elevated cysteine-rich 61 (CYR61) and monocyte chemoattractant protein-1 (MCP-1) via action of transcription factor AP-1. Collagen is degraded by MMP-1 activity but synthesized by transforming growth factor-β (TGF-β) signal. Chlorella has been shown to inhibit UVB-induced MMP-1 level, however its regulatory molecular mechanisms have not been studied. In this study, Chlorella derived peptide (CDP) was added to skin fibroblasts after UVB irradiation and the expression of MMP-1, CYR61, procollagen, c-fos, c-jun, and TGF-β receptor (TbRII) mRNA and MCP-1 production were investigated. CDP (10 or 5 mg/ml) diminished UVB-induced MMP-1 and CYR61 mRNA expression and MCP-1 production, whereas, UVB-suppressed procollagen and TbRII mRNA was restored by CDP treatment. UVB-induced c-fos and c-jun expressions were also inhibited by the CDP treatment. Taken together, CDP inhibits UVB-induced MMP-1 expression in skin fibroblasts by suppressing expression of AP-1 and CYR61 and MCP-1 production.
Relation:	Regulatory Toxicology and Pharmacology, 60(1), pp.112-119
Appears in Collections:	[Dept. of Pharmacy] Periodical Articles

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