Mutational Analysis of Splicing Activities of Ribonucleotide Reductase α Subunit Protein from Lytic Bacteriophage P1201

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標題:	Mutational Analysis of Splicing Activities of Ribonucleotide Reductase α Subunit Protein from Lytic Bacteriophage P1201
作者:	Kan, Shu-Chen Yu, Liang-Kun Chen, Jiau-Hua Hu, Hui-Yu Hsu, Wen-Hwei
貢獻者:	食品科技系
日期:	2011-04
上傳時間:	2013-06-26 15:34:39 (UTC+8)
出版者:	Springer-Verlag
摘要:	A CP1201 RIR1 intein is found in the ribonucleotide reductase alpha subunit (RNR α subunit) protein of lytic bacteriophage P1201 from Corynebacterium glutamicum NCHU 87078. This intein can be over-expressed and spliced in Escherichia coli NovaBlue cells. Mutations of C539, the N-terminal residue of the C-extein in the CP1201 RIR1 protein, led to the changes of pattern and level of protein-splicing activities. A G392S variant was found to be a temperature-sensitive protein with complete splicing activity at 17 and 28°C but not at 37°C or higher. We also found that the cleavage at the CP1201 RIR1 intein C-terminus of the double mutant G392S/C539G was blocked, but other cleavage activities could be efficiently performed at 17°C. G392S/C539G variant possessed the properties of low-temperature-induced cleavage at the intein N-terminus.
關聯:	Current Microbiology 62(4), pp.1282-1286
显示于类别:	[ 食品科技系 ] 期刊論文

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