Inhibitory Effects of Polygonum cuspidatum on the Epstein-Barr virus lytic cycle

CNU IR > Pharmacy and Science > Dept. of Health and Nutrition (including master's program) > Periodical Articles > Item 310902800/26733

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://ir.cnu.edu.tw/handle/310902800/26733

题名:	Inhibitory Effects of Polygonum cuspidatum on the Epstein-Barr virus lytic cycle
作者:	YIU, CHING-YI CHEN, SHIH-YING HUANG, CHING-WEN YEH, DONG-BOR LIN, TSUEY-PIN
贡献者:	保健營養系(所)
关键词:	Polygonum cuspidatum
日期:	2011-06
上传时间:	2013-06-26 11:37:01 (UTC+8)
摘要:	Polygonum cuspidatum is widely used as a medicinal herb in Asia. In this study, we examined the ethanolic extract of Polygonum cuspidatum roots (PcE) for their capacity to inhibit the EBV lytic cycle. The cell viability was determined by the MTT [3-(4,5- dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide] method. The expression of Epstein-Barr virus (EBV) lytic proteins was analyzed by flow cytometric assay. Real-time quantitative PCR was used to measure the EBV virion production. Transient transfection analysis was subsequently performed to assess the transcription of lytic genes, including BRLF1 and BZLF1. Results showed that the PcE inhibited the transcription of EBV immediate early genes, the expression of EBV lytic proteins, including Rta, Zta, and EA-D and reduced the production of virus particles, showing that PcE is useful for preventing the proliferation of the virus.
關聯:	藥物食品分析19(2), pp.107-113
显示于类别:	[Dept. of Health and Nutrition (including master's program)] Periodical Articles

文件中的档案:

档案	描述	大小	格式	浏览次数
index.html		0Kb	HTML	3392	检视/开启

在CNU IR中所有的数据项都受到原著作权保护.

TAIR相关文章