探索Rath細胞穿透性胜肽在細胞內的傳遞

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Title:	探索Rath細胞穿透性胜肽在細胞內的傳遞 Exploration of Rath cell-penetrating peptide in intracellular delivery
Authors:	林佳緯
Contributors:	藥物科技研究所郭榮華
Keywords:	細胞穿透性胜肽 Rath胜肽共價結合 FITC U-937 HeLa Cell penetrating peptide Rath peptide conjugat
Date:	2012
Issue Date:	2013-04-22 16:49:59 (UTC+8)
Abstract:	對使用細胞穿透性胜肽 (CPPs) 或是蛋白質轉導區域當作在細胞內的傳遞上載體感興趣的越來越多。細胞穿透性胜肽，單獨使用或是再結合各種被傳遞分子，可以跨越生物膜的屏障，而且對細胞的生物膜沒有顯著的破壞以及很低的細胞毒性。在目前所廣泛使用的細胞穿透性胜肽中，由禽流感病毒所衍生的Rath胜肽，已經被證實具有細胞穿透性胜肽的特性。 Rath胜肽可以非共價鍵與被傳遞分子相互作用並具有輸送大型分子至細胞內。因此，本研究的目的是來探索Rath胜肽在細胞內傳遞的特性化研究。 Rath胜肽及其結合FITC標記的Rath胜肽，進行了生物相容性的測試，並且確定最佳劑量的範圍。同時，在N-端結合FITC標記的Rath胜肽細胞攝取高於在C-端結合FITC標記的Rath胜肽。我們發現，Rath胜肽能夠傳遞FITC到U-937及HeLa細胞中。 Interest in using cell-penetrating peptides (CPPs) or protein transduction domains as carriers for intracellular delivery is increasing. Cell-penetrating peptides, alone or coupled with various cargo molecules, can cross biological membrane barriers without significantly damaging the membranes and with little cytotoxicity to the cells. Of the cell-penetrating peptides used, Rath peptide, derived from the avian infectious bursal disease virus, has been identified. Rath peptide is capable of non-covalent interaction and delivering large cargo to primary cells. Therefore, the aim of this study is to characterize and explore of Rath peptide in intracellular delivery. Biocompatibility of Rath peptide and its conjugation of FITC (fluorescein-5-isothiocynate) were tested and the optimal dose ranges were determined. Also, Cell uptake of Rath conjugated FITC in N-terminus was higher than that in C-terminus. We found that Rath peptide is capable of delivering FITC to U-937 and HeLa cells.
Relation:	校內校外均不公開，學年度：100，42頁
Appears in Collections:	[Dept. of Pharmacy] Dissertations and Theses

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